Method for determining at least one hemoglobin related parameter of a whole blood sample
First Claim
1. A method for determining at least one hemoglobin related parameter of a whole blood sample, comprising the steps ofdepositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber;
- imaging the at least one red blood cell contacting the interior surfaces, and producing image signals;
determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces using the image signals; and
determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.
1 Assignment
0 Petitions
Accused Products
Abstract
A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.
115 Citations
13 Claims
-
1. A method for determining at least one hemoglobin related parameter of a whole blood sample, comprising the steps of
depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; -
imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces using the image signals; and determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
-
13. A method for determining at least one hemoglobin related parameter of a whole blood sample, comprising the steps of:
-
depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; imaging the at least one red blood cell and producing image signals using a transmittance light source; determining an optical density of at least a portion of the imaged red blood cell using the image signals; and determining the at least one hemoglobin related parameter of the red blood cell, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin.
-
Specification