Extraction of polar lipids by a two solvent method
First Claim
1. A method of separating polar lipids and proteins from intact algal cells, comprising:
- providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids;
dewatering the wet algal biomass by removing extracellular water to increase the solid content of the wet algal biomass to between 5% and 50% to result in a partially dewatered algal biomass comprising intact algal cells;
mixing the partially dewatered wet algal biomass with an amphipathic solvent set in a ratio of about one part amphipathic solvent set to about one part partially dewatered wet algal biomass by weight, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the water, amphipathic solvent set, partially dewatered wet algal biomass that comprises intact algal cells, and proteins that are soluble in a mixture of an amphipathic solvent and water, and the lighter phase comprises the hydrophobic solvent set and polar lipids;
separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture;
separating the polar lipids from the lighter phase to generate a polar lipids fraction;
separating the partially dewatered wet algal biomass from the heavier phase of the extraction mixture to generate a protein mixture comprising proteins that are soluble in a mixture of an amphipathic solvent and water, water, and the amphipathic solvent set; and
separating the proteins of the protein mixture from at least a portion of the water and amphipathic solvent to generate a protein fraction, wherein the protein mixture has a higher concentration of proteins that are soluble in a mixture of an amphipathic solvent and water than the separated partially dewatered wet algal biomass.
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Abstract
A method for separating polar lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. Neutral lipids remaining in the algal biomass after extraction of polar lipids can be used to generate renewable fuels.
174 Citations
20 Claims
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1. A method of separating polar lipids and proteins from intact algal cells, comprising:
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providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids; dewatering the wet algal biomass by removing extracellular water to increase the solid content of the wet algal biomass to between 5% and 50% to result in a partially dewatered algal biomass comprising intact algal cells; mixing the partially dewatered wet algal biomass with an amphipathic solvent set in a ratio of about one part amphipathic solvent set to about one part partially dewatered wet algal biomass by weight, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the water, amphipathic solvent set, partially dewatered wet algal biomass that comprises intact algal cells, and proteins that are soluble in a mixture of an amphipathic solvent and water, and the lighter phase comprises the hydrophobic solvent set and polar lipids; separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture; separating the polar lipids from the lighter phase to generate a polar lipids fraction; separating the partially dewatered wet algal biomass from the heavier phase of the extraction mixture to generate a protein mixture comprising proteins that are soluble in a mixture of an amphipathic solvent and water, water, and the amphipathic solvent set; and separating the proteins of the protein mixture from at least a portion of the water and amphipathic solvent to generate a protein fraction, wherein the protein mixture has a higher concentration of proteins that are soluble in a mixture of an amphipathic solvent and water than the separated partially dewatered wet algal biomass. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of separating polar lipids and proteins from intact algal cells, comprising:
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providing a wet algal biomass comprising intact algal cells which comprise water, proteins that are soluble in a mixture of an amphipathic solvent and water, and polar lipids; mixing the wet algal biomass with an amphipathic solvent set in a ratio of about one part amphipathic solvent to about one part partially dewatered wet algal biomass by weight, and a hydrophobic solvent set to generate an extraction mixture comprising a heavier phase and a lighter phase, wherein the heavier phase comprises the water, amphipathic solvent set, wet algal biomass that comprises intact algal cells, and proteins that are soluble in a mixture of an amphipathic solvent and water, and the lighter phase comprises the hydrophobic solvent set and polar lipids; separating the heavier phase of the extraction mixture from the lighter phase of the extraction mixture; separating the polar lipids from the lighter phase to generate a polar lipids fraction; separating the wet algal biomass from the heavier phase of the extraction mixture to generate a protein mixture comprising proteins that are soluble in a mixture of an amphipathic solvent and water, water, and amphipathic solvent set; and separating the proteins of the protein mixture to generate a protein fraction, wherein the protein mixture has a higher concentration of proteins that are soluble in a mixture of an amphipathic solvent and water than the separated partially dewatered wet algal biomass.
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Specification