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Methods and compositions for tagging and identifying polynucleotides

  • US 8,476,018 B2
  • Filed: 05/08/2012
  • Issued: 07/02/2013
  • Est. Priority Date: 02/10/2005
  • Status: Active Grant
First Claim
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1. A method of estimating the number of target polynucleotides in a sample, comprising:

  • ligating a discriminatory oligonucleotide tag to a complex nucleic acid sample comprising multiple molecular species so that substantially every target polynucleotide receives a tag that comprises a unique nucleotide sequence, to produce a tagged sample;

    amplifying the target polynucleotides in said tagged sample; and

    determining the number of different tags that are present in the amplified target polynucleotides, thereby providing an estimate of the number of target polynucleotides that are amplified.

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