Method of making an array of nucleic acid colonies
First Claim
1. A method of making an array of nucleic acid colonies, comprising(a) providing a substrate comprising a patterned surface of features, wherein the features are spatially separated from each other on the surface of the substrate;
- (b) contacting the substrate with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, wherein a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution;
(c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the subset; and
(d) repeating steps (b) and (c) to increase the number of the features on the surface that comprise a nucleic acid colony, thereby making an array of nucleic acid colonies.
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Abstract
A method of making an array of nucleic acid colonies, by (a) providing a substrate having a patterned surface of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the substrate with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, wherein a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the subset; and (d) repeating steps (b) and (c) to increase the number of the features on the surface that have a nucleic acid colony, thereby making an array of nucleic acid colonies.
71 Citations
19 Claims
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1. A method of making an array of nucleic acid colonies, comprising
(a) providing a substrate comprising a patterned surface of features, wherein the features are spatially separated from each other on the surface of the substrate; -
(b) contacting the substrate with a solution of different target nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, wherein a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the subset; and (d) repeating steps (b) and (c) to increase the number of the features on the surface that comprise a nucleic acid colony, thereby making an array of nucleic acid colonies. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification