Sucrose feedstock utilization for oil-based fuel manufacturing
First Claim
1. A method of cultivating microalgae Chlorella or Prototheca in a culture medium, the microalgae comprising an exogenous gene encoding a secretable sucrose invertase, the method comprising the steps of:
- (a) providing the microalgae;
(b) adding sucrose to the culture medium; and
(c) cultivating the microalgae in the culture medium, wherein the invertase enzyme allows the microalgae to grow on sucrose.
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Abstract
The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar beets, and cane/beet agricultural processing byproducts as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a conversion technology to convert chemical energy produced by sugar cane and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oil-bearing microbes containing one or more exogenous sucrose utilization genes.
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Citations
25 Claims
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1. A method of cultivating microalgae Chlorella or Prototheca in a culture medium, the microalgae comprising an exogenous gene encoding a secretable sucrose invertase, the method comprising the steps of:
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(a) providing the microalgae; (b) adding sucrose to the culture medium; and (c) cultivating the microalgae in the culture medium, wherein the invertase enzyme allows the microalgae to grow on sucrose. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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22. A method of cultivating microalgae Chlorella or Prototheca in a culture medium that comprises sucrose, the method comprising the steps of:
- (a) providing the microalgae comprising an exogenous secretable sucrose invertase gene; and
(b) cultivating the microalgae in said culture medium, wherein the culture medium comprises limited nitrogen and wherein the invertase enzyme allows the microalgae to grow on sucrose. - View Dependent Claims (23, 24)
- (a) providing the microalgae comprising an exogenous secretable sucrose invertase gene; and
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25. A method for producing triglycerides, the method comprising:
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(a) heterotrophically cultivating a population of microalgae Chlorella or Prototheca on a sucrose medium, the microalgae expressing a transgene encoding a secreted invertase enzyme and a secretion signal such that the invertase enzyme hydrolyzes sucrose in the medium and allows the microalgae to grow on sucrose; and (b) recovering the triglycerides.
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Specification