Cassette for sample preparation
First Claim
Patent Images
1. A method, comprising:
- moving a plunger within a reagent chamber of a cartridge such that the plunger punctures a portion of a puncturable member defining a boundary of the reagent chamber, the moving the plunger including transferring a plurality of magnetic particles from the reagent chamber to a first chamber defined by a housing of the cartridge;
disposing a magnetic shaft at least partially within a movable valve member of a valve to attract the plurality of magnetic particles into a cavity defined by the movable valve member, the valve at least partially disposed within the first housing, the valve including the movable valve member and a valve housing;
rotating the movable valve member relative to the valve housing to transfer the plurality of magnetic particles between the first chamber of the housing and a second chamber defined by the housing while sealing the plurality of magnetic particles within the cavity;
disposing the magnetic shaft at least partially within a movable valve member of a second valve to attract the plurality of magnetic particles into a cavity defined by the movable valve member of the second valve, the second valve at least partially disposed within the housing, the second valve including the movable valve member and a valve housing; and
moving the valve member of the second valve relative to the valve housing of the second valve to transfer the plurality of magnetic particles between the second chamber of the housing and a third chamber defined by the housing.
4 Assignments
0 Petitions
Accused Products
Abstract
A cassette for preparing a sample is disclosed herein. The cassette includes a housing, which encloses the structures and the processes used to prepare the sample.
111 Citations
25 Claims
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1. A method, comprising:
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moving a plunger within a reagent chamber of a cartridge such that the plunger punctures a portion of a puncturable member defining a boundary of the reagent chamber, the moving the plunger including transferring a plurality of magnetic particles from the reagent chamber to a first chamber defined by a housing of the cartridge; disposing a magnetic shaft at least partially within a movable valve member of a valve to attract the plurality of magnetic particles into a cavity defined by the movable valve member, the valve at least partially disposed within the first housing, the valve including the movable valve member and a valve housing; rotating the movable valve member relative to the valve housing to transfer the plurality of magnetic particles between the first chamber of the housing and a second chamber defined by the housing while sealing the plurality of magnetic particles within the cavity; disposing the magnetic shaft at least partially within a movable valve member of a second valve to attract the plurality of magnetic particles into a cavity defined by the movable valve member of the second valve, the second valve at least partially disposed within the housing, the second valve including the movable valve member and a valve housing; and moving the valve member of the second valve relative to the valve housing of the second valve to transfer the plurality of magnetic particles between the second chamber of the housing and a third chamber defined by the housing. - View Dependent Claims (2, 3, 4, 5)
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6. A method for isolating nucleic acids from a sample comprising:
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(a) receiving a sample in a mixing chamber of a cassette, the cassette comprising the mixing chamber, a first washing chamber, a first valve comprising a valve housing defining a first valve opening in fluid communication with the mixing chamber and a second valve opening in fluid communication with the first washing chamber, a second valve comprising a valve housing defining a first valve opening in fluid communication with the first washing chamber and a second valve opening in fluid communication with a second washing chamber, a first reagent chamber configured to contain a lysis buffer, a second reagent chamber configured to contain magnetic particles, and a third reagent chamber configured to contain a binding buffer, wherein the first, second, and third reagent chambers each include a puncturable portion defining at least a portion of a boundary between the mixing chamber and the first, second, and third reagent chambers; (b) transferring the lysis buffer to the mixing chamber by puncturing the puncturable portion of the first reagent chamber; (c) transferring the magnetic particles to the mixing chamber by puncturing the puncturable portion of the second reagent chamber; (d) transferring the binding buffer to the mixing chamber by puncturing the puncturable portion of the third reagent chamber; (e) binding nucleic acids in the sample to the magnetic particles; (f) receiving the magnetic particles and the bound nucleic acids in a cavity defined by a valve member disposed in the valve housing; (g) transferring the magnetic particles and the bound nucleic acids from the mixing chamber to the first washing chamber by moving the valve member relative to the valve housing from a first position in which the cavity is in fluid communication with the mixing chamber to a second position in which the cavity is in fluid communication with the washing chamber. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification