Conditioned cell culture medium compositions and methods of use
First Claim
1. A method of making a composition comprising:
- (a) culturing mesenchymal cells on microcarriers in a cell culture medium until the cell culture medium comprises at least 3.2 ng/mL vascular endothelial growth factor;
(b) removing the medium from the cell culture; and
(c) combining the medium with a pharmaceutically acceptable carrier to form the composition.
3 Assignments
0 Petitions
Accused Products
Abstract
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic cell type. Once the cell medium of the invention is conditioned, it may be used in any state. Physical embodiments of the conditioned medium include, but are not limited to, liquid or solid, frozen, lyophilized or dried into a powder. Additionally, the medium is formulated with a pharmaceutically acceptable carrier as a vehicle for internal administration, applied directly to a food item or product, or formulated with a salve or ointment for topical applications. Also, the medium may be further processed to concentrate or reduce one or more factors or components contained within the medium.
53 Citations
20 Claims
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1. A method of making a composition comprising:
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(a) culturing mesenchymal cells on microcarriers in a cell culture medium until the cell culture medium comprises at least 3.2 ng/mL vascular endothelial growth factor; (b) removing the medium from the cell culture; and (c) combining the medium with a pharmaceutically acceptable carrier to form the composition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A composition comprising a medium prepared by:
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(a) culturing mesenchymal cells on microcarriers in a cell culture medium until the cell culture medium comprises at least 3.2 ng/mL vascular endothelial growth factor; (b) removing the medium from the cell culture; and (c) combining the medium with a pharmaceutically acceptable carrier to form the composition. - View Dependent Claims (12, 13)
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14. A method of making a composition comprising:
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(a) inoculating microcarriers with mesenchymal cells in a cell culture medium in vitro; (b) allowing the microcarriers to be joined by cells and/or extracellular matrix; (c) removing the medium from the cell culture; and (d) combining the medium with a pharmaceutically acceptable carrier to form the composition. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification