Methods and apparatus for biomedical agent multi-dimension measuring and analysis

• US 8,477,307 B1
• Filed: 01/26/2012
• Issued: 07/02/2013
• Est. Priority Date: 01/26/2012
• Status: Expired due to Fees

• Current Assignee: Ann Rachel Yufa
• Original Assignee: Ann Rachel Yufa
• Inventors: Yufa, Ann Rachel, Yufa, Aleksandr Leybovich
• Primary Examiner(s): Ton, Tri T
• Application Number:
  US13/374,976
• Time in Patent Office: 523 Days
• Field of Search: 356335-343, 356 27- 285, 356 301- 515, 356432-444, 34087001-87017
• US Class Current: 356/338
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2021/1734 Sequential different kinds of measurements; Combining two or more methods
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids

Abstract

This invention, methods and apparatus for biomedical agent multi-dimension measuring and analysis, provide the multi-dimension measuring of the biomedical agents, cells, molecules, any and all explosive and hazardous agents, aerosol, airborne particles, liquid/fluid contaminations/particles, gas particles, etc. in order to recognize the multi-dimension size of the particles, select and sort them by their multi-dimension measurements, and count the particles in each sorted group. An improved apparatus for biomedical agent multi-dimension measuring and analysis comprises at least two light (laser) beam sources and at least two light detection means respectively located in the chamber. Also, the improved apparatus includes the amplifying, processing and control means providing distinguish and imaging of the particles/biomedical agents by their multi-dimensions, thereby, providing more precise agent grouping for analysis and counting.

Claims

1. A method for biomedical agent multi-dimension measuring and analysis comprising the steps of

providing at least two light beam sources of a plurality of light beam sources, wherein a first light beam source provides a first light beam along a first light beam axis and a second light beam source provides a second light beam along a second light beam axis;

providing a flow of said biomedical agent along a biomedical agent flow axis through a biomedical agent monitoring region;

providing a first intersection of said biomedical agent flow axis with said first light beam axis at a first point on said biomedical agent flow axis within said biomedical agent monitoring region;

providing a second intersection of said biomedical agent flow axis with said second light beam axis at a second point on said biomedical agent flow axis within said biomedical agent monitoring region, wherein a location of said second point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis;

providing a detection of a light, which is created during said first intersection, by said first light detection means, wherein said light is a scattered light created by said biomedical agent flowing through said first point located within said biomedical agent monitoring region;

providing a detection of a light, which is created during said second intersection, by said second light detection means, wherein said light is said scattered light created by said biomedical agent flowing through said second point located within said biomedical agent monitoring region;

providing by said first light detection means an output which is effectively indicative of a first dimension of said biomedical agent flowing through said first point;

providing by said second light detection means an output which is effectively indicative of a second dimension of said biomedical agent flowing through said second point;

processing the outputs from said first light detection means and from said second light detection means providing said multi-dimension measuring of said biomedical agent and said analysis of said biomedical agent, wherein said analysis includes at least a counting of the biomedical agents, defining a concentration of the biomedical agents, a radioactive absorption testing, a X-Ray absorption testing, an infrared testing, an ultraviolet testing, a magnetic testing, an electromagnetic testing, an electromagnetic radiation absorption testing, a mass spectroscopy testing, a liquid chromatography testing, a flame ionization testing, a chemical testing, a DNA melting point testing, a DNA titration testing, an electrochemical testing, a fluorescent chemical testing, a fluorescence quenching testing, a redox-labeled nucleic acid binding testing, an oscillatory spectral detection testing.

2. The method of claim 1, wherein further said first light beam source provides a first scanning light beam which provides a scanning of each biomedical agent flowing through said first point within said biomedical agent monitoring region and said second light beam source provides a second scanning light beam which provides said scanning of said each biomedical agent flowing through said second point within said biomedical agent monitoring region, and wherein said scanning by said first scanning light beam of each biomedical agent flowing through said first point within said biomedical agent monitoring region and said scanning by said second scanning light beam of said each biomedical agent flowing through said second point within said biomedical agent monitoring region are provided in a synchronized sequence.

3. The method of claim 1, wherein further said method provides at least three light beam sources of a plurality of light beam sources, wherein said first light beam source provides said first light beam along said first light beam axis, said second light beam source provides said second light beam along said second light beam axis and a third light beam source provides a third light beam along a third light beam axis;

providing a third intersection of said biomedical agent flow axis with said third light beam axis at a third point on said biomedical agent flow axis within said biomedical agent monitoring region, wherein a location of said third point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis and from location of said second point on said biomedical agent flow axis;

providing a detection of a light, which is created during said third intersection, by a third light detection means, wherein said light is a scattered light created by said biomedical agent flowing through said third point located within said biomedical agent monitoring region;

providing by said third light detection means an output which is effectively indicative of a third dimension of said biomedical agent flowing through said third point;

processing the outputs from said first light detection means, from said second light detection means and from third light detection means providing said multi-dimension measuring of said biomedical agent and said analysis of said biomedical agent, wherein said analysis includes at least a counting of the biomedical agents, defining of a concentration of the biomedical agents, a radioactive absorption testing, a X-Ray absorption testing, an infrared testing, an ultraviolet testing, a magnetic testing, an electromagnetic testing, an electromagnetic radiation absorption testing, a mass spectroscopy testing, a liquid chromatography testing, a flame ionization testing, a chemical testing, a DNA melting point testing, a DNA titration testing, an electrochemical testing, a fluorescent chemical testing, a fluorescence quenching testing, a redox-labeled nucleic acid binding testing, an oscillatory spectral detection testing.

4. The method of claim 3, wherein further said first light beam source provides a first scanning light beam which provides a scanning of each biomedical agent flowing through a first point within said biomedical agent monitoring region, said second light beam source provides a second scanning light beam which provides said scanning of said each biomedical agent flowing through a second point within said biomedical agent monitoring region and said third light beam source provides a third scanning light beam which provides said scanning of said each biomedical agent flowing through said third point located within said biomedical agent monitoring region, and wherein said scanning by said first scanning light beam of each biomedical agent flowing through said first point within said biomedical agent monitoring region, said scanning by said second scanning light beam of said each biomedical agent flowing through said second point within said biomedical agent monitoring region and said scanning by said third scanning light beam of each biomedical agent flowing through said third point within said biomedical agent monitoring region are provided in a synchronized sequence.

5. An apparatus for biomedical agent multi-dimension measuring and analysis comprising

a first light emitting-detecting portion including

a first light beam source, providing a first scanning light beam, wherein said first scanning light beam provides a first intersection of a biomedical agent flow axis with said first scanning light beam at a first point on said biomedical agent flow axis within a biomedical agent monitoring region;

a first light detection means connected to a light detection signal amplifying means, wherein said first light detection means provides an output which is effectively indicative of a first dimension of said multi-dimension measuring;

a second light emitting-detecting portion including

a second light beam source, providing a second scanning light beam, wherein said second scanning light beam provides a second intersection of said biomedical agent flow axis with said second scanning light beam at a second point on said biomedical agent flow axis within said biomedical agent monitoring region, and wherein a location of said second point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis;

a second light detection means connected to said light detection signal amplifying means, wherein said second light detection means provides an output which is effectively indicative of a second dimension of said multi-dimension measuring;

a third light emitting-detecting portion including

a third light beam source, providing a third scanning light beam, wherein said third scanning light beam provides a third intersection of said biomedical agent flow axis with said third scanning light beam at a third point on said biomedical agent flow axis within said biomedical agent monitoring region, and wherein location of said third point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis and from location of said second point on said biomedical agent flow axis;

a third light detection means connected to said light detection signal amplifying means, providing an amplification of the detected signals from said first light detection means, from said second light detection means and from said third light detection means, wherein said third light detection means provides an output which is effectively indicative of a third dimension of said multi-dimension measuring;

a free-surface fluidic means coupled with a biomedical agent flow tubular means by a specimen delivery means and coupled at least with a surface enhanced raman scattering means which is connected to said light detection signal amplifying means which amplifies the detection signals from said surface enhanced raman scattering means, with a radioactive radiation analysis means, with an infrared analysis means, with a chemical analysis means, with an electrochemical analysis means, with a magnetic radiation analysis means, with an electromagnetic radiation analysis means, with a radioactive absorption analysis means, with a X-Ray absorption analysis means, with an ultraviolet analysis means, with an electromagnetic radiation absorption analysis means, with a spectroscopic analyzing means, with a mass spectroscopy analysis means, with a liquid chromatography analysis means, with a flame ionization analysis means, with a DNA melting point analyzing means, with a DNA titration analysis means, with a fluorescence quenching analysis means, with a redox-labeled nucleic acid binding analysis means, with an oscillatory spectral detection analysis means, providing said analysis;

an amplified signal processing means, providing an initial processing of the amplified signals, connected to a biomedical agent form recognition means, to a biomedical analytical means, summarizing the analyzed information from the connected analysis means, and to said light detection signal amplifying means, wherein said biomedical analytical means is connected at least to said radioactive radiation analysis means, to said infrared analysis means, to said chemical analysis means, to said electrochemical analysis means, to said magnetic radiation analysis means, to said electromagnetic radiation analysis means, to said radioactive absorption analysis means, to said X-Ray absorption analysis means, to said ultraviolet analysis means, to said electromagnetic radiation absorption analysis means, to said spectroscopic analysis means, to said mass spectroscopy analysis means, to said liquid chromatography analysis means, to said flame ionization analysis means, to said DNA melting point analysis means, to said DNA titration analysis means, to said fluorescence quenching analysis means, to said redox-labeled nucleic acid binding analysis means, and to said oscillatory spectral detection analysis means;

a biomedical agent form imaging means connected to said biomedical agent recognition means which combines said first dimension, said second dimension and said third dimension into an information about said multi-dimension measuring, and selects and sorts an identical measurement information about biomedical agents in the groups with the identical three-dimension measurement, wherein said biomedical agent recognition means provides a counting of the biomedical agents in each size group or provides said analysis of a concentration of the biomedical agents, and wherein said biomedical agent form imaging means provides an initial processing of the scanned information and the information from said biomedical agent recognition means;

an information processing, control and displaying means, providing a final processing of the combined information, connected to said biomedical analytical means and to said biomedical agent form imaging means, wherein said information processing, control and displaying means comprises at least a microprocessing means and a terminal means including a display means, a floppy disk means, a memory, a printing means, a control panel, and compact disk means which are connected to each other by a multiplexed bus or by a data bus and an address bus, wherein said microprocessing means of said information processing, control and displaying means, said biomedical analytical means, said biomedical agent form imaging means, said biomedical agent form recognition means, and said amplified signal processing means are connected to each other and to said terminal means by said multiplexed bus or by said data bus and said address bus.

6. The apparatus of claim 5, wherein further said biomedical agent recognition means provides said counting of the biomedical agents in each size group and provides said analysis of said concentration of the biomedical agents displaying means.