SPR apparatus with a high performance fluid delivery system

• US 8,477,313 B2
• Filed: 11/30/2012
• Issued: 07/02/2013
• Est. Priority Date: 12/18/2007
• Status: Active Grant

• Current Assignee: Suzhou Puxin Life Science & Technology Limited
• Original Assignee: Plexera, LLC
• Inventors: Kim, Gilbum, Guan, Hannwen, Cong, Shuxin
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US13/691,472
• Publication Number:
  US 20130088717A1
• Time in Patent Office: 214 Days
• Field of Search: 356445-448
• US Class Current: 356/445
• CPC Class Codes: G01N 21/01 Arrangements or apparatus for facilitating the optical investigation
• CPC Class Codes: G01N 21/11 Filling or emptying of cuvettes
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

An apparatus may include a flow cell for a surface plasmon resonance test apparatus and a fluid delivery system operatively coupled to the flow cell and configured to deliver a plurality of fluids to the flow cell substantially continuously. The fluid delivery system may include two pumps for pumping the fluids.

Claims

1. A surface plasmon resonance (SPR) system, comprising:

a microarray;

a SPR coupling surface adjacent to the microarray;

an SPR optical subsystem;

a flow cell; and

a fluid delivery system comprising;

an injection valve including two or more ports;

at least one holding coil; and

one or more pumps in communication with one or more of the injection valve, the holding coil, and the flow cell.

2. The SPR system of claim 1, where the SPR optical subsystem is configured to illuminate the SPR coupling surface and detect changes in light reflected from locations on the microarray.

3. The SPR system of claim 1, where a fluid is continuously pumped through the flow cell.

4. The SPR system of claim 1, where the injection valve includes a first port coupled to receive an analyte solution injected into the at least one holding coil and one or more ports coupled to receive fluid pushed by the one or more pumps.

5. The SPR system of claim 4, where the injection valve is configured to switch between two positions, where the first position allows the analyte solution to be injected into the holding coil, and the second position allows the one or more pumps to pump the analyte solution into the flow cell.

6. The SPR system of claim 5, where in the first position a fluid is pumped through the flow cell from a first pump.

7. The SPR system of claim 5, where in the second position a fluid is pumped through the holding coil into the flow cell from a second pump.

8. The SPR system of claim 1, where the fluid delivery system includes tubing having hydrophilic surfaces.

9. The SPR system of claim 8, where the tubing includes one or both silica-coated surfaces and tubing treated with a surfactant.

10. The SPR system of claim 1, where the flow cell has one or more internal hydrophilic surfaces.

11. The SPR system of claim 1, where the injection valve is configured to couple the first pump to the holding coil.

12. The SPR system of claim 1, where the injection valve is configured to couple the second pump to the holding coil.

13. The SPR system of claim 11, further comprising a selection valve configured to selectively couple the first pump to one or both a plurality of fluid sources and the injection valve.

14. The SPR system of claim 13, where at least one of the plurality of fluid sources includes an auto sampler.

15. The SPR system of claim 13, further comprising a controller operatively coupled to the selection valve.

16. The SPR system of claim 4, further comprising a controller configured to prevent the one or more pumps from being contaminated by the analyte solution.

17. The SPR system of claim 1, where the fluid delivery system further includes a buffer solution and at least one regeneration fluid.

18. A surface plasmon resonance (SPR) system, comprising:

a flow cell including a microarray in communication with a fluid delivery system comprising;

an injection valve including one or more ports;

at least one holding coil; and

one or more pumps in communication with one or more of the injection valve, the holding coil, and the flow cell;

a SPR coupling surface adjacent to the microarray; and

an SPR optical subsystem.

19. The SPR system of claim 18, where the SPR optical subsystem includes an illuminator, a prism adjacent to the SPR coupling surface and a detector.

20. The SPR system of claim 18, further comprising a controller and an external computing system which control the fluid delivery system.