Method of measuring electrical resistance value of corneal trans-epithelium

• US 8,478,395 B2
• Filed: 03/03/2009
• Issued: 07/02/2013
• Est. Priority Date: 03/03/2008
• Status: Active Grant

• Current Assignee: Nagasaki University, Osaka University
• Original Assignee: Nagasaki University, Tohoku University National Universities Corporation
• Inventors: Nishizawa, Matsuhiko, Kaji, Hirokazu, Sekine, Soichiro, Uematsu, Masafumi, Kitaoka, Takashi, Nishida, Koji, Tanaka, Yuji
• Primary Examiner(s): Dougherty, Sean P
• Assistant Examiner(s): Stout, Michael C
• Application Number:
  US12/921,069
• Publication Number:
  US 20110046509A1
• Time in Patent Office: 1,582 Days
• Field of Search: 600/547, 600/558
• US Class Current: 600/547
• CPC Class Codes: A61B 2562/0215 Silver or silver chloride containing
• CPC Class Codes: A61B 5/053 Measuring electrical impedance or conductance of a portion of the body
• CPC Class Codes: A61B 5/6821 Eye

Abstract

The invention provides a method for evaluating a corneal disorder quantitatively and is applicable to living eyes. In particular, the invention provides a method for measuring a corneal transepithelial electric resistance, which method comprises: (1) a step of placing a first electrode on the cornea and a second electrode on the conjunctiva; and (2) a step of flowing an electric current between the first electrode and the second electrode to measure the electric resistance. The invention also provides a device for measuring a corneal transepithelial electric resistance value.

Claims

1. A method for measuring a corneal transepithelial electric resistance value, comprising

(1) placing electrodes on a cornea and a conjunctiva, wherein the electrodes are connected to a voltage-electric resistance value measuring device, and wherein the device flows an electric current through corneal epithelium, and

(2) measuring the corneal transepithelial electric resistance value based on the electric current flowing through the corneal epithelium.

3. The method according to claim 1, wherein a periphery of the electrode placed on the cornea is insulated.

4. The method according to claim 1 which is carried out on a living eye.

10. The method according to claim 3 which is carried out on a living eye.

2. A method for measuring a corneal transepithelial electric resistance value comprising:

(1) a step of preparing a voltage-electric resistance value measuring device, and a first electrode and a second electrode which are connected to the device;

(2) a step of placing the first electrode on the cornea and the second electrode on the conjunctiva; and

(3) a step of measuring the corneal transepithelial electric resistance value by flowing an electric current between the first electrode and the second electrode and through corneal epithelium to measure an electric resistance.

11. The method according to claim 2, wherein a periphery of the electrode placed on the cornea is insulated.

12. The method according to claim 2 which is carried out on a living eye.

5. A device for measuring a corneal transepithelial electric resistance on a living eye, which comprises:

(1) a first electrode suitable to be placed on a cornea,

(2) a second electrode suitable to be placed on a conjunctiva, ˜

d

(3) an insulator that insulates the first electrode, and

(4) a voltage-electric resistance value measuring device connected to the first electrode and the second electrode, wherein the device is capable of flowing an electric current through corneal epithelium,

wherein the first electrode and the insulator are integrated, and

wherein the insulator is made of silicone rubber, and the insulator has a contact surface to the cornea having the same curvature as that of the cornea.

6. The device according to claim 5, wherein the first electrode and the insulator are integrated via a conductor.

7. The device according to claim 5, wherein the second electrode is further integrated.

8. The device according to claim 5, wherein the second electrode is formed into a sheet shape.

9. The device according to claim 6, wherein the conductor is a gel of hyaluronic acid or atelocollagen.