Biosensor, thin film electrode forming method, quantification apparatus, and quantification method

• US 8,480,866 B2
• Filed: 01/20/2011
• Issued: 07/09/2013
• Est. Priority Date: 11/15/1999
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Miyazaki, Shoji, Yamanishi, Eriko
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US12/930,919
• Publication Number:
  US 20110117269A1
• Time in Patent Office: 901 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

Claims

1. A method of manufacturing a biosensor comprising the steps of:

roughening the surface of an insulating support by exposing the surface of the insulating support to an excited gas in a vacuum chamber,

forming a thin film electrode layer on the roughened surface of the insulating support, wherein the thin film electrode layer has a roughened surface that reflects the roughened surface of the underlying insulating support; and

forming a reagent layer on the thin film electrode layer.

2. The method of manufacturing a biosensor of claim 1, wherein the thin film electrode layer comprises a conductive substance selected from the group consisting of noble metal and carbon.

3. The method of manufacturing a biosensor of claim 2, wherein the thin film electrode layer has a thickness from 3 nm to 100 nm.

4. The method of manufacturing a biosensor of claim 3, wherein the reagent layer comprises an electron carrier.

5. The method of manufacturing a biosensor of claim 2, wherein the reagent layer comprises an electron carrier.

6. The method of manufacturing a biosensor of claim 1, wherein the thin film electrode layer has a thickness from 3 nm to 100 nm.

7. The method of manufacturing a biosensor of claim 6, wherein the reagent layer comprises an electron carrier.

8. The method of manufacturing a biosensor of claim 1, wherein the reagent layer comprises an electron carrier.

9. The method of manufacturing a biosensor of claim 1, wherein the roughening step comprises colliding the excited gas against the surface of the insulating support.

10. A method of manufacturing a biosensor, comprising the steps of:

roughening a surface of an insulating support;

forming an electrode on the roughened surface of the insulating support, wherein the electrode has a roughened surface that reflects the roughened surface of the underlying insulating support; and

forming a reagent layer on the electrode.

11. The method of manufacturing a biosensor of claim 10, wherein the reagent layer is on the electrode and on at least a portion of the insulating support.

12. The method of manufacturing a biosensor of claim 10, wherein the surface of the insulating support is roughened by exposing the surface to an excited gas.

13. A method of manufacturing a biosensor, comprising the steps of:

placing an insulating support in a vacuum chamber which is filled with inert gas and applying a voltage thereto;

forming an electrode on a surface of the insulating support, wherein the electrode has a surface that reflects the surface of the underlying insulating support; and

forming a reagent layer on the electrode.

14. The method of manufacturing a biosensor of claim 13, wherein the inert gas is selected from the group consisting of argon, neon, helium, krypton, xenon and nitrogen.