Biosensor, thin film electrode forming method, quantification apparatus, and quantification method

• US 8,480,867 B2
• Filed: 07/15/2011
• Issued: 07/09/2013
• Est. Priority Date: 11/15/1999
• Status: Expired due to Fees

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Miyazaki, Shoji, Fujiwara, Masaki, Tokuno, Yoshinobu
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/135,848
• Publication Number:
  US 20110278167A1
• Time in Patent Office: 725 Days
• Field of Search: 20440301-40315, 204/406, 205/777.5, 205/778, 205/792
• US Class Current: 204/403.02
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/48771 Coding of information, e.g. calibration data, lot number
• CPC Class Codes: Y10T 29/49002 Electrical device making

Abstract

Apparatus are disclosed for measuring substrate content present in a specimen that is applied to a biosensor, where the apparatus include an input for receiving an electrical signal from the biosensor; and a circuit coupled to the input to detect the electrical signal to determine whether an amount of the specimen needed for specimen detection has been supplied to the biosensor; where the apparatus measures the substrate content included in the specimen when the circuit determines that an amount of the specimen needed for specimen detection has been supplied to the biosensor, and the apparatus is prevented from measuring the substrate content included in the specimen when the circuit determines that the specimen has not been sufficiently supplied to the biosensor.

Claims

1. An apparatus for measuring substrate content present in a specimen that is applied to a biosensor, the apparatus comprising:

an input for receiving an electrical signal from the biosensor; and

a circuit coupled to the input to detect the electrical signal to determine whether an amount of the specimen needed for specimen detection has been supplied to the biosensor;

wherein;

the apparatus measures the substrate content included in the specimen when the circuit determines that an amount of the specimen needed for specimen detection has been supplied to the biosensor, and

the apparatus has circuitry to prevent it from measuring the substrate content included in the specimen when the circuit determines that the specimen has not been sufficiently supplied to the biosensor, even if additional specimen is thereafter added to the biosensor.

2. The apparatus of claim 1, wherein the circuit determines whether an amount of the specimen needed for specimen detection has been supplied to the biosensor based on the presence of the electrical signal.

3. The apparatus of claim 2, wherein the circuit determines that an insufficient amount of the specimen has been supplied to the biosensor based on the absence of the electrical signal.

4. The apparatus of claim 3, further comprising a display part coupled to the circuit, wherein the display part shows that the specimen has not been sufficiently supplied to the biosensor.

5. The apparatus of claim 4, wherein the circuit operates to prevent the apparatus from measuring the substrate content included in the specimen after the display part shows that the specimen has not been sufficiently supplied to the biosensor.

6. An apparatus for measuring substrate content in a specimen using a biosensor that includes a first pair of electrodes and a second pair of electrodes in a specimen supply path, the apparatus comprising:

(a) connectors to the electrodes of the biosensor;

(b) a current/voltage conversion circuit that is connected to the connectors;

(c) an analog/digital (A/D) conversion circuit that is connected to the current/voltage conversion circuit;

(d) a central processing unit (CPU) for calculating the amount of substrate included in the specimen based on pulses from the A/D conversion circuit; and

(e) a display for displaying a measured value calculated by the CPU;

wherein the CPU is configured for;

(i) a first detecting step for detecting an electrical change in the first pair of electrodes when the specimen is applied to the biosensor;

(ii) a second detecting step for detecting an electrical change in the second pair of electrodes after detecting the first electrical change;

(iii) a judging step for judging a shortage of specimen amount needed for measurement, when an electrical change is not detected in the second detecting step within a prescribed period of time after the first electrical change;

(iv) an indicating step for indicating to a user that an amount of specimen needed for measurement is not sufficiently supplied to the specimen supply path if a shortage of the specimen amount is judged in the judging step; and

(v) a stopping step for stopping measurement of substrate content based on a shortage of specimen amount as judged by the judging step;

wherein the CPU is configured to stop measurement of substrate content even if additional specimen is added to the specimen supply path after the judging step.