Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
First Claim
1. A biosensor for quantifying a substrate in a sample liquid comprising:
- an insulating support;
a working electrode formed from an electrically conductive layer that is made of a noble metal and is provided on a surface of the insulating support;
a reagent layer on the working electrode;
a spacer layer provided on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and
a cover provided on the spacer layer, where the cover comprises a counter electrode formed from an electrically conductive layer that is made of a noble metal and is provided on an inner surface of the cover;
wherein a portion of the counter electrode and a portion of the working electrode are in the supply path, andwherein part of the reagent layer is in the supply path.
5 Assignments
1 Petition
Accused Products
Abstract
A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
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Citations
38 Claims
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1. A biosensor for quantifying a substrate in a sample liquid comprising:
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an insulating support; a working electrode formed from an electrically conductive layer that is made of a noble metal and is provided on a surface of the insulating support; a reagent layer on the working electrode; a spacer layer provided on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and a cover provided on the spacer layer, where the cover comprises a counter electrode formed from an electrically conductive layer that is made of a noble metal and is provided on an inner surface of the cover; wherein a portion of the counter electrode and a portion of the working electrode are in the supply path, and wherein part of the reagent layer is in the supply path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A biosensor for quantifying a substrate in a sample liquid comprising:
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an insulating support; an electrically conductive layer that is made of a noble metal and is provided on a surface of the insulating support; an electrode pattern comprising at least a working electrode and a counter electrode, where the electrode pattern is formed from the electrically conductive layer; a slit provided on the electrically conductive layer between the working electrode and the counter electrode, where the slit has a depth that is equal to or greater than the thickness of the electrically conductive layer; a reagent layer on the electrode pattern; a spacer layer provided on and covering part of the reagent layer, where the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer and where the supply path formed by the spacer layer is narrower than the reagent layer; and a cover provided on the spacer layer; wherein a portion of the counter electrode and a portion of the working electrode are in the supply path, and wherein part of the reagent layer is in the supply path. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification