Detection of degradation products of feline NT-proBNP

• US 8,481,333 B2
• Filed: 05/31/2012
• Issued: 07/09/2013
• Est. Priority Date: 05/31/2011
• Status: Active Grant

• Current Assignee: IDEXX Laboratories Incorporated
• Original Assignee: IDEXX Laboratories Incorporated
• Inventors: Yerramilli, Mahalakshmi, Atkinson, Michael, Yerramilli, Murthy V. S. N.
• Primary Examiner(s): Cheu, Jacob
• Assistant Examiner(s): BELEI, CARMENCITA
• Application Number:
  US13/485,286
• Publication Number:
  US 20120329175A1
• Time in Patent Office: 404 Days
• Field of Search: None
• US Class Current: 436/517
• CPC Class Codes: G01N 2333/58 Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
• CPC Class Codes: G01N 2800/32 Cardiovascular disorders
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors

Abstract

A method for determining the amount of NT-proBNP in blood samples from felines. The method includes detecting degradation products of feline NT-proBNP by various methods, including using antibodies, kits and devices.

Claims

1. A kit for determining the presence, absence or level of feline NT-proBNP in a sample comprising:

(a) a solid support;

(b) a first antibody, the first antibody being specific for a first epitope on a fragment of feline NT-proBNP, the fragment being less than the full length of feline NT-proBNP and being stable in feline serum or plasma at room temperature after at least 192 hours from taking the sample wherein the fragment of feline NT-proBNP is selected from the group consisting of ASEASAIQELLDGLRDTVSEL (SED ID NO;

5);

LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;

9) and

LQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;

10); and

(c) a second antibody being specific for a second epitope on the fragment of feline NT-proBNP, the second epitope being different than the first epitope.

2. An immunoassay device comprising:

(a) a solid support having a first antibody bound thereto, the first antibody being specific for a first epitope on a fragment of feline NT-proBNP;

(b) a fragment of feline NT-proBNP being bound to the first antibody, the fragment being less than the full length of feline NT-proBNP and being stable in feline serum or plasma at room temperature after at least 192 hours from taking the sample wherein the fragment of feline NT-proBNP is selected from the group consisting of ASEASAIQELLDGLRDTVSEL (SED ID NO;

5);

LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;

9) and

LQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;

10); and

(c) a second antibody being specific for a second epitope on the fragment of feline NT-proBNP, the second epitope being different than the first epitope, the second antibody being bound to the fragment of feline NT-proBNP.