Detection of degradation products of feline NT-proBNP
First Claim
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1. A kit for determining the presence, absence or level of feline NT-proBNP in a sample comprising:
- (a) a solid support;
(b) a first antibody, the first antibody being specific for a first epitope on a fragment of feline NT-proBNP, the fragment being less than the full length of feline NT-proBNP and being stable in feline serum or plasma at room temperature after at least 192 hours from taking the sample wherein the fragment of feline NT-proBNP is selected from the group consisting of ASEASAIQELLDGLRDTVSEL (SED ID NO;
5);
LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
9) andLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
10); and
(c) a second antibody being specific for a second epitope on the fragment of feline NT-proBNP, the second epitope being different than the first epitope.
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Abstract
A method for determining the amount of NT-proBNP in blood samples from felines. The method includes detecting degradation products of feline NT-proBNP by various methods, including using antibodies, kits and devices.
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Citations
2 Claims
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1. A kit for determining the presence, absence or level of feline NT-proBNP in a sample comprising:
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(a) a solid support; (b) a first antibody, the first antibody being specific for a first epitope on a fragment of feline NT-proBNP, the fragment being less than the full length of feline NT-proBNP and being stable in feline serum or plasma at room temperature after at least 192 hours from taking the sample wherein the fragment of feline NT-proBNP is selected from the group consisting of ASEASAIQELLDGLRDTVSEL (SED ID NO;
5);LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
9) andLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
10); and(c) a second antibody being specific for a second epitope on the fragment of feline NT-proBNP, the second epitope being different than the first epitope.
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2. An immunoassay device comprising:
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(a) a solid support having a first antibody bound thereto, the first antibody being specific for a first epitope on a fragment of feline NT-proBNP; (b) a fragment of feline NT-proBNP being bound to the first antibody, the fragment being less than the full length of feline NT-proBNP and being stable in feline serum or plasma at room temperature after at least 192 hours from taking the sample wherein the fragment of feline NT-proBNP is selected from the group consisting of ASEASAIQELLDGLRDTVSEL (SED ID NO;
5);LGPLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
9) andLQQGHSPAESWEAQEEPPARVLAPHDNVLR (SEQ ID NO;
10); and(c) a second antibody being specific for a second epitope on the fragment of feline NT-proBNP, the second epitope being different than the first epitope, the second antibody being bound to the fragment of feline NT-proBNP.
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Specification