Compositions and methods for measuring 3,6-L-AHG transferase activity and 3,6-L-AHG

• US 8,486,654 B2
• Filed: 02/24/2011
• Issued: 07/16/2013
• Est. Priority Date: 06/15/2010
• Status: Expired due to Fees

• Current Assignee: Korea University Research and Business Foundation (Korea University), Samsung Electronics Co. Ltd.
• Original Assignee: Korea University Research and Business Foundation (Korea University), Samsung Electronics Co. Ltd.
• Inventors: Park, Sung Min, Park, Jae Chan, Choi, In Geol, Koo, Hyun Min, Kim, Kyoung Heon
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): YAMASAKI, ROBERT J
• Application Number:
  US13/034,387
• Publication Number:
  US 20110306059A1
• Time in Patent Office: 873 Days
• Field of Search: None
• US Class Current: 435/26
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: G01N 2333/91102 with definite EC number (2.4.1.-)

Abstract

Provided herein are a composition for measuring 3,6-anhydro-L-galactose (3,6-L-AHG) transferase activity by reduction of NADP to NADPH, and a method of measuring 3,6-L-AHG transferase activity using the same. The composition and method are useful for determining 3,6-L-AHG in a material containing 3,6-L-AHG such as algae biomass and industrial applications.

Claims

1. A method of measuring enzymatic modification of 3,6-anhydro-L-galactose (3,6-L-AHG), comprising:

contacting an enzyme extract with a composition comprising NADP⁺, 3,6-L-AHG, and a buffer; and

measuring the amount of NADPH produced.

2. The method of claim 1, wherein amount of NADPH is determined by measuring absorbance at a wavelength of about 339 to 340 nm.