Arrays of magnetic particles

• US 8,486,720 B2
• Filed: 08/03/2004
• Issued: 07/16/2013
• Est. Priority Date: 06/21/2000
• Status: Active Grant

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Banerjee, Sukanta, Seul, Michael, Li, Alice X.
• Primary Examiner(s): Shibuya, Mark
• Assistant Examiner(s): DO, PENSEE T
• Application Number:
  US10/910,460
• Publication Number:
  US 20050272049A1
• Time in Patent Office: 3,269 Days
• Field of Search: 436/526, 436/518, 436/525, 435/287.2, 435/526, 435/518, 435/525
• US Class Current: 436/526
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00545 Colours
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2563/143 Magnetism, e.g. magnetic label
• CPC Class Codes: C12Q 2563/155 : Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2565/513 : characterised by the pattern of the arrayed oligonucleotides
• CPC Class Codes: G01N 2035/00564 : Handling or washing solid phase elements, e.g. beads
• CPC Class Codes: G01N 2035/00762 : magnetic code
• CPC Class Codes: G01N 2446/20 : the magnetic material being present in the particle core
• CPC Class Codes: G01N 2446/64 : Magnetic material dispersed in oil drop
• CPC Class Codes: G01N 27/745 : for detecting magnetic beads used in biochemical assays concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098
• CPC Class Codes: G01N 33/5434 : using magnetic particle immunoreagent carriers which constitute new materials per se
• CPC Class Codes: G01N 33/54346 : Nanoparticles
• CPC Class Codes: G01N 33/587 : Nanoparticles
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis

Abstract

The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.

Claims

1. A method of forming an array, comprising:

providing a plurality of magnetic particles comprising at least two different particle populations, each bearing a different binding agent and being distinguishable by an optically differentiable code associated therewith, suspended in a fluid;

contacting the magnetic particles with analytes in solution under conditions permitting the binding agents to bind to analytes in an analyte-specific manner;

assembling the magnetic particles, following said contacting and binding to analytes, in a planar array on one or more chips using a magnetic field; and

then forming a gel film on the surface of the chips and immobilizing the magnetic particles on the chips by embedding them in the gel film.

2. The method of claim 1 wherein the particles are randomly placed in the array.

3. The method of claim 1 wherein a plurality of arrays are assembled on a plurality of chips.

4. The method of claim 2 wherein the chips include microwells patterned on the chips.

5. The method of claim 1 wherein the gel film is a polymer.