Fluorescence emissions detector

• US 8,487,272 B2
• Filed: 12/14/2010
• Issued: 07/16/2013
• Est. Priority Date: 12/14/2010
• Status: Active Grant

• Current Assignee: Authentix, Inc.
• Original Assignee: Authentix, Inc.
• Inventors: Tinsley, Kenneth E.
• Primary Examiner(s): Sung, Christine
• Application Number:
  US12/967,579
• Publication Number:
  US 20120145924A1
• Time in Patent Office: 945 Days
• Field of Search: 250/458.1, 250/459.1, 250/461.1
• US Class Current: 250/458.1
• CPC Class Codes: G01J 1/46 using a capacitor
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: H03F 3/10 with diodes parametric amplifiers H03F7/00

Abstract

A light source is gated ON and OFF in response to a pulsed signal. Photo emissions from the light source are coupled to a material under test. Resonant fluorescent emissions from the material are coupled to a photodiode. Current from the photodiode is coupled into an amplifier system comprising a first and second amplifier stages. The first amplifier stage is gated to a low gain when the light source is turned ON and the gain is increased when the light source goes from ON to OFF. The second amplifier stage has digitally programmable offset and gain settings in response to control signals. The output of the second amplifier stage is digitized by an analog to digital converter. A controller generates the pulse control signal and the control signals.

Claims

1. A system for detecting resonant fluorescence comprising:

a light emitting diode (LED) configured to emit pulsed photoemissions towards a target in response to a pulsed signal;

a photodiode configured to (1) receive resonant fluorescence emissions from the target, the resonant fluorescence emissions a response to the pulsed photoemissions, and (2) generate a current in response to the received resonant fluorescence emissions; and

an amplifier system having first and second stages, wherein the first stage is configured to receive (1) the current from the photodiode, and (2) a gating signal as a function of the pulsed signal, the first stage further configured so that the gating signal gates the first stage to have a first output with (1) a first gain when the LED is activated to emit one of the pulsed photoemissions, and (2) a second gain when the LED is deactivated, wherein the second stage is configured to receive control signals and the first output of the first stage, the second stage having a second output and having a gain and offset configured to respond to the control signals to produce a linear dynamic range for a signal at the second output of the second stage that is an analog of the resonant fluorescence emissions from the target.

2. The system of claim 1 further comprising a controller programmed to generate the pulsed signal and the control signals.

3. The system of claim 1 further comprising an analog-to-digital converter for digitizing the second output of the second stage.

4. The system of claim 1, wherein the first stage comprises a transimpedance amplifier receiving the current and generating a voltage proportional to the current.

5. The system of claim 4, wherein the first stage comprises a first operational amplifier having a positive input selectively coupled to a ground potential through a first resistor/capacitor network setting a first input source impedance, the photodiode coupled between a negative input of the first operational amplifier and a supply voltage, and a second resistor/capacitor network selectively coupled from the negative input to an output of the first operational amplifier setting the first gain of the first stage and a second input source impedance.

6. The system of claim 5, wherein a first analog switch selectively couples resistors of the first resistor/capacitor network to the positive input of the first operational amplifier in response to the pulsed signal.

7. The system of claim 5, wherein a second analog switch selectively couples resistors of the second resistor/capacitor network to the negative input of the first operational amplifier in response to the pulsed signal thereby decreasing the first gain during stimulated emissions from the LED and increasing the first gain during resonance fluorescence emissions from the target.

8. The system of claim 1, wherein the second stage comprises a second operational amplifier having a positive input coupled to the first output of the first stage, a negative input coupled through a first resistor to an offset voltage and a three terminal voltage divider network including selectively coupled parallel resistors connected in series with a single resistor, one terminal of the voltage divider network is coupled to the second output of the second stage, a second terminal of the voltage divider network is coupled to a ground potential and a third terminal of the voltage divider network is coupled to the negative input of the second operational amplifier.

9. The system of claim 8, wherein the parallel resistor network comprises analog switches for selectively connecting resistors of the parallel resistor network to the negative terminal of the second operational amplifier, the analog switches selectable in response to the control signals.

10. The system of claim 8 further comprising an analog-to-digital converter having an input coupled to the second output of the second stage and digital outputs generating a digital signal.

11. The system of claim 8 further comprising a programmable offset voltage generator having an output generating the offset voltage in response to the control signals.

12. The system of claim 6, wherein the resistors are selectively connected in parallel with the capacitor in first resistor/capacitor network in response to the control signals and the values of the resistors and capacitor are sized to minimize the differences in impedances seen by charge injection at the inputs of the first operational amplifier when the gain of the first amplifier stage is gated by the pulse signal thus minimizing response time of the system.

13. The system of claim 12, wherein the output of the offset generator is isolated from the first resistor with a low output impedance buffer stage.

14. The system of claim 8, wherein the offset generator is programmed with parallel resistor network selectively connected in a voltage divider network in response to the control signals.

15. A method for detecting resonant fluorescent emissions from a target, comprising:

irradiating the target with light from a pulsed light source, thereby causing the target to emit resonant fluorescent emissions, the pulsed light source receiving a signal that activates and de-activates the pulsed light source to gate the light ON and OFF;

receiving the resonant fluorescent emissions from the target by a photoconductor to thereby produce a current modulated by the received resonant fluorescent emissions;

coupling the current from the photoconductor to an amplifier system comprising a first stage amplifier that converts the current to a voltage output and a second stage amplifier with programmable gain and offset that receives the voltage output and produces an amplifier output;

reducing a gain and balancing source impedances of the first stage amplifier in response to the signal that activates the pulsed light source ON; and

increasing the gain and balancing source impedances of the first stage amplifier in response to the signal deactivating the pulsed light source from ON to OFF.

16. The method of claim 15 further comprising:

analyzing the amplifier output for cut-off and saturation;

optimizing a dynamic range of the amplifier system;

adjusting digitally the programmable gain of the second stage amplifier to optimize the dynamic range, and

adjusting digitally the offset of the second stage amplifier to optimize the dynamic range.

17. The method of claim 16 further comprising converting the amplifier output to a digital signal using an analog-to-digital converter.

18. The method of claim 17, further comprising storing the digital signal in a controller as an analog of the resonant fluorescent emission.

19. The method of claim 15, wherein the photoconductor is a photodiode.

20. The method of claim 15, wherein the balancing of source impedances of the first stage amplifier minimizes a settling time of the amplifier system.