Tissue engineered blood vessels
First Claim
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1. A method for producing a preconditioned blood vessel, comprising:
- providing a biocompatible matrix shaped in a tubular configuration seeded with a cultured population of endothelial cells on the inside surface of the matrix, wherein the tubular matrix comprises at least one nanoparticle coupled to a releasable therapeutic agent which is incorporated within the tubular matrix;
attaching a first and second end of the tubular matrix to a first and second attachment element in a preconditioning chamber, wherein the first and second attachment elements have a channel that is fluidly coupled to a fluid flow system; and
preconditioning the seeded tubular matrix with the flow system by moving a biological fluid through the seeded tubular matrix, wherein the flow-rate and pulse-rate of the biological fluid is controlled such that the preconditioned blood vessel is produced.
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Abstract
The invention is directed to methods for preparing artificial blood vessels by preconditioning a matrix seeded with endothelial cells to fluid flow conditions that mimic blood flow.
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23 Claims
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1. A method for producing a preconditioned blood vessel, comprising:
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providing a biocompatible matrix shaped in a tubular configuration seeded with a cultured population of endothelial cells on the inside surface of the matrix, wherein the tubular matrix comprises at least one nanoparticle coupled to a releasable therapeutic agent which is incorporated within the tubular matrix; attaching a first and second end of the tubular matrix to a first and second attachment element in a preconditioning chamber, wherein the first and second attachment elements have a channel that is fluidly coupled to a fluid flow system; and preconditioning the seeded tubular matrix with the flow system by moving a biological fluid through the seeded tubular matrix, wherein the flow-rate and pulse-rate of the biological fluid is controlled such that the preconditioned blood vessel is produced. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification