Marker of wound infection

• US 8,491,852 B2
• Filed: 04/24/2006
• Issued: 07/23/2013
• Est. Priority Date: 05/20/2005
• Status: Expired due to Fees

• Current Assignee: Woundchek Laboratories (US), Inc.
• Original Assignee: Systagenix Wound Management (US), Inc.
• Inventors: Cullen, Breda Mary, Clark, Rachael, Stephens, Philip, Moseley, Ryan
• Primary Examiner(s): COLE, MONIQUE T
• Application Number:
  US11/914,884
• Publication Number:
  US 20090299161A1
• Time in Patent Office: 2,647 Days
• Field of Search: 422/55, 422/56, 422/58, 422/99, 422/104, 422/430, 422/420, 422/408, 436/62, 436/63, 436/69, 436/810
• US Class Current: 422/430
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/528 Atypical element structures, e.g. gloves, rods, tampons, toilet paper
• CPC Class Codes: G01N 33/6893 related to diseases not provided for elsewhere

Abstract

An indicator device adapted give a detectable signal when the antioxidant capacity of a sample of a mammalian wound fluid exceeds a predetermined minimum level which is characteristic of an infected wound. Also provided system for use in the diagnosis and treatment of wounds comprising the inventive diagnostic device and a wound dressing comprising at least one antimicrobial agent for selective application to infected wounds. Also provided are methods of diagnosis and treatment by means of the inventive device and system.

Claims

1. A system for use in the diagnosis and treatment of wounds comprising a diagnostic device, the diagnostic device comprising an indicator device adapted to give a detectable signal when the antioxidant capacity of a sample of a mammalian wound fluid exceeds a predetermined minimum level which is characteristic of an infection, the indicator device comprising a housing containing one or more reagents, the housing having at least one inlet provided therein for introduction of the sample, wherein the indicator device comprises a source of at least one reactive oxygen species and an indicator molecule that reacts with the reactive oxygen species to produce a detectable change, and a wound dressing comprising at least one antimicrobial agent, the wound dressing to be applied to the wound when the measured total antioxidant capacity is indicative of an infected wound.

2. The system for use in the diagnosis and treatment of wounds of claim 1 comprising a diagnostic device, wherein the indicator device comprises a redox indicator molecule selected from the group consisting of Cytochrome C, 1,10-phenanthroline iron salt, diphenylamine sulfonic acid, triphenylmethane dyes, starch-iodine, diphenylpicryihydrazyl (DPPH) and 2,2′

-azino-di(3-ethylbenzthiazoline sulfonate) (ABTS).

3. The system for use in the diagnosis and treatment of wounds of claim 1 comprising a diagnostic device, wherein the indicator device comprises an electrochemical sensor.

4. The system for use in the diagnosis and treatment of wounds of claim 1 comprising a diagnostic device, wherein the indicator device comprises a wound dressing, dipstick or swab.

5. The system for use in the diagnosis and treatment of wounds of claim 1 comprising a diagnostic device, which is a lateral flow diagnostic device.

6. The system for use in the diagnosis and treatment of wounds of claim 1 comprising a diagnostic device, wherein the indicator device is adapted to detect at least one further analyte in addition to the total antioxidant capacity of the sample.

7. The system for use in the diagnosis and treatment of wounds of claim 6 comprising a diagnostic device, wherein the at least one further analyte comprises total protein content of the sample.

8. A system for use in the diagnosis and treatment of wounds comprising a diagnostic device, the diagnostic device comprising an indicator device adapted to give a detectable signal when the antioxidant capacity of a sample of a mammalian wound fluid exceeds a predetermined minimum level which is characteristic of an infection, the indicator device comprising a housing containing one or more reagents, the housing having at least one inlet provided therein for introduction of the sample, wherein the indicator device comprises a source of at least one reactive oxygen species and an indicator molecule that reacts with the reactive oxygen species to produce a detectable change, and a wound dressing to be applied to the wound when the measured total antioxidant capacity is indicative of a non-infected wound.

9. The system for use in the diagnosis and treatment of wounds of claim 8 comprising a diagnostic device, wherein the device comprises a redox indicator molecule selected from the group consisting of Cytochrome C, 1,10-phenanthroline iron salt, diphenylamine sulfonic acid, triphenylmethane dyes, starch-iodine, diphenylpicryihydrazyl (DPPH) and 2,2′

-azino-di(3-ethylbenzthiazoline sulfonate) (ABTS).

10. The system for use in the diagnosis and treatment of wounds of claim 8 comprising a diagnostic device, wherein the device comprises an electrochemical sensor.

11. The system for use in the diagnosis and treatment of wounds of claim 8 comprising a diagnostic device, wherein the device comprises a wound dressing, dipstick or swab.

12. The system for use in the diagnosis and treatment of wounds of claim 8 comprising a diagnostic device, which is a lateral flow diagnostic device.

13. The system for use in the diagnosis and treatment of wounds of claim 8 comprising a diagnostic device, wherein the device is adapted to detect at least one further analyte in addition to the total antioxidant capacity of the sample.

14. The system for use in the diagnosis and treatment of wounds of claim 13 comprising a diagnostic device, wherein the at least one further analyte comprises total protein content of the sample.