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Subtractive single label comparative hybridization

  • US 8,492,089 B2
  • Filed: 02/16/2011
  • Issued: 07/23/2013
  • Est. Priority Date: 07/30/2007
  • Status: Expired due to Fees
First Claim
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1. A method of detecting aneuploid cells in a sample, comprising:

  • amplifying nucleic acid from a test sample suspected of comprising aneuploid cells and amplifying nucleic acid from a reference sample, wherein one of the amplification reactions is conducted using dUTP and not dTTP and the other is conducted using dTTP and not dUTP;

    hybridizing to a nucleic acid array a solution comprising the amplified test sample and amplified reference sample; and

    determining the relative amount of hybridized test and reference nucleic acids bound to the array, wherein a difference in the relative amount of hybridized test and reference nucleic acids bound to the array identifies the presence of aneuploid cells;

    wherein the sample amplified using dUTP is degraded.

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