Droplet-based affinity assays

• US 8,492,168 B2
• Filed: 12/15/2006
• Issued: 07/23/2013
• Est. Priority Date: 04/18/2006
• Status: Expired due to Fees

• Current Assignee: Duke University, Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Srinivasan, Vijay, Pamula, Vamsee K., Pollack, Michael G., Fair, Richard B.
• Primary Examiner(s): Shibuya, Mark
• Assistant Examiner(s): DO, PENSEE T
• Application Number:
  US11/639,663
• Publication Number:
  US 20070275415A1
• Time in Patent Office: 2,412 Days
• Field of Search: 436/526, 335/26
• US Class Current: 436/526
• CPC Class Codes: B01F 33/3021 the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
• CPC Class Codes: B01F 33/3031 using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/148 Specific details about calibrations
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 2400/0442 thermal energy, e.g. vaporisation, bubble jet
• CPC Class Codes: B01L 2400/0448 Marangoni flow; Thermocapillary effect
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502784 : specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/525 : with physical movement of samples between temperature zones
• CPC Class Codes: G01N 15/1484 : microstructural devices
• CPC Class Codes: G01N 2015/1006 : for cytology
• CPC Class Codes: G01N 33/54373 : involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.

Claims

1. A method of detecting a target analyte in a sample, the method comprising:

(a) executing electrowetting-mediated droplet operations using droplets on a droplet microactuator to effect an immunoassay, wherein the electrowetting-mediated droplet operations are selected to;

(i) combine one or more immunoassay reagent droplets comprising an antibody immobilized on one or more magnetically-responsive beads disposed in a droplet with a sample droplet potentially comprising the target analyte to yield a combined droplet, wherein the one or more immunoassay reagent droplets, the sample droplet, and the combined droplet are each;

(1) arranged on the droplet microactuator; and

(2) subject to the electrowetting-mediated droplet operations; and

(ii) effect a droplet-based washing protocol on the droplet microactuator using a droplet comprising the magnetically responsive beads, wherein the washing protocol comprises conducting droplet operations selected to;

(1) merge one or more wash buffer droplets with a droplet comprising the magnetically responsive beads; and

(2) split the droplet comprising the magnetically responsive beads, while using a magnet to retain the magnetically responsive beads, thereby removing a sub-droplet comprising unbound substances from a sub-droplet comprising the beads; and

(b) generating and detecting a signal which corresponds to the presence, absence and/or quantity of the analyte in the sample.

2. The method of claim 1 wherein the detecting step comprises detecting a signal from a droplet on a droplet microactuator.

3. The method of claim 1 wherein the signal measured corresponds to unoccupied binding sites of the antibody immobilized on the one or more magnetically responsive beads.

4. The method of claim 1 wherein the signal measured corresponds to occupied binding sites of the antibody immobilized on the one or more magnetically responsive beads.

5. The method of claim 1 wherein the immunoassay reagents comprise a labeled analyte tracer.

6. The method of claim 5 wherein the labeled tracer generates a signal only when bound to the immunoassay reagent.

7. The method of claim 5 wherein the labeled tracer generates a signal only when not bound to the immunoassay reagent.

8. The method of claim 1 wherein the electrowetting-mediated droplet operations are selected to effect a competitive immunoassay protocol.

9. The method of claim 1 wherein the immunoassay reagents comprise an antibody which is selective for a target temperature zone analyte.

10. The method of claim 1 wherein the immunoassay reagents comprise two or more antibodies, each selective for a specific epitope or region of the target analyte.

11. The method of claim 1 wherein the immunoassay reagents comprise two or more antibodies, including a first antibody selective for the target analyte and a second antibody selective for the first antibody.

12. The method of claim 1 wherein the immunoassay reagents comprise a labeled antibody.

13. The method of claim 12 wherein the labeled antibody comprises a radioactive isotope.

14. The method of claim 12 wherein the electrowetting-mediated droplet operations further comprise electrowetting-mediated droplet operations to effect a droplet-based washing protocol for separating labeled antibody which is bound to the analyte from labeled antibody which is not bound to the analyte.

15. The method of claim 1 wherein the immunoassay reagents comprise an antibody conjugated to an enzyme capable of catalyzing a detectable reaction.

16. The method of claim 15 wherein the detectable reaction results in a colorimetric, fluorescent and/or luminescent property or signal.

17. The method of claim 1 wherein the immunoassay reagents comprise reagents for conducting an assay type selected from the group consisting of sandwich assays, immunometric assays, immunometric assays, capture bridge assays, direct class capture assays, indirect class capture assays, assays for small molecules, noncompetitive assays for small molecules, ambient analyte assays and free analyte assays.

18. The method of claim 17 comprising conducting two or more of the assay types simultaneously on a droplet microactuator.

19. The method of claim 17 comprising conducting three or more of the assay types simultaneously on a droplet microactuator.

20. The method of claim 1 further comprising conducting electrowetting-mediated droplet operations to develop a calibration curve.

21. The method of claim 1 wherein:

(a) the immunoassay reagent droplets comprise a labeled detecting antibody;

(b) the droplet operations merge one or more droplets comprising the antibody immobilized on one or more magnetically-responsive beads, the labeled detecting antibody, and the sample droplet to yield, in the presence of the analyte, a magnetically responsive bead-antibody-analyte-detecting antibody complex;

(c) the unbound substances comprise unreacted components of the sample and/or unreacted excess detecting antibody; and

(d) the detecting step comprises detecting a signal from the magnetically responsive bead-antibody-analyte-detecting antibody complex.

22. The method of claim 1 wherein the electrowetting-mediated droplet operations employ a sample from a source selected from the group consisting of whole blood, lymphatic fluids, serum, plasma, sweat, tear, saliva, sputum, cerebrospinal fluids, amniotic fluids, seminal fluids, vaginal excretions, serous fluids, synovial fluids, pericardial fluids, peritoneal fluids, pleural fluids, transudates, exudates, cystic fluids, bile, urine, gastric fluids, intestinal fluids, fecal samples, fluidized tissues, fluidized organisms, biological swabs and biological washes.

23. The method of claim 1 wherein the electrowetting-mediated droplet operations further comprise contacting an antibody in a droplet with a magnetically responsive bead in a droplet to result in immobilization of the antibody on the magnetically responsive bead.

24. The method of claim 23 wherein the contact is achieved by droplet operations comprising combining the droplet comprising the antibody with a droplet comprising a bead.

25. The method of claim 1 wherein the electrowetting-mediated droplet operations further comprise conducting an electrode-mediated droplet-based washing protocol on the droplet microactuator to remove excess detection antibody from the magnetically responsive beads.

26. The method of claim 1 wherein the electrowetting-mediated droplet operations further comprise providing the sample as a sample droplet on the droplet actuator.

27. The method of claim 1 wherein the electrowetting-mediated droplet operations further comprise exposing the antibody immobilized on one or more magnetically-responsive beads to a droplet including a reporter antibody.

28. The method of claim 27 wherein the electrowetting-mediated droplet operations further comprise washing away excess reporter antibody.

29. The method of claim 1 wherein the signal comprises an electrochemical property of a droplet on a droplet microactuator.

30. The method of claim 29 wherein the electrochemical property comprises an amperometric, potentiometric and/or conductometric property of a droplet on the droplet microactuator.

31. The method of claim 1 wherein the signal comprises an optical property of a droplet on a droplet microactuator.

32. The method of claim 31 wherein the optical property comprises absorbance, chemiluminescence and/or fluorescence.

33. The method of claim 31 wherein the optical property comprises Raman spectra.

34. The method of claim 1 wherein the signal comprises a physical parameter of a droplet on a droplet microactuator.

35. The method of claim 34 wherein the physical parameter comprises temperature.

36. The method of claim 34 wherein the physical parameter comprises emission of radioactive particles.

37. A method of detecting a target analyte in a sample, the method comprising executing the following electrowetting-mediated droplet operations on a droplet microactuator:

(a) merging a droplet comprising the magnetically responsive beads with a sample droplet potentially including the target analyte with binding affinity for the immobilized antibody and yielding, in the presence of a target analyte, an immobilized antibody-target analyte complex;

(b) washing the immobilized antibody-target analyte complex thereby removing unbound components of the sample droplet, wherein the washing comprises;

(i) merging one or more wash droplets with a droplet comprising the one or more magnetically responsive beads and unbound components; and

(ii) splitting a droplet comprising the one or more magnetically responsive beads and unbound components, while using a magnet to immobilize the magnetically responsive beads, thereby yielding a sub-droplet comprising the magnetically responsive beads and a sub-droplet substantially lacking the magnetically responsive beads;

(c) merging a droplet comprising the magnetically responsive beads and washed antibody-target analyte complex with a droplet including a reporter antibody;

(d) washing away excess reporter antibody, wherein the washing comprises;

(i) merging one or more wash droplets with a droplet comprising the one or more magnetically responsive beads and excess reporter antibody; and

(ii) splitting a droplet comprising the one or more magnetically responsive beads and excess reporter antibody while magnetically retaining the magnetically responsive beads, thereby yielding a sub-droplet comprising the magnetically responsive beads and a sub-droplet substantially lacking the magnetically responsive beads; and

(e) performing additional steps to produce a measurable parameter or signal.