Integrated system and process for bioproduct production

• US 8,497,105 B2
• Filed: 06/24/2010
• Issued: 07/30/2013
• Est. Priority Date: 06/26/2009
• Status: Expired due to Fees

• Current Assignee: Talfryn SA
• Original Assignee: Cobalt Technologies, Inc.
• Inventors: Walther, David C., Meerman, Hendrik J., Burns-Guydish, Stacy M., Wilson, Richard W., Hogg, Eamon T., Luli, Gregory W., Eckert, Robert
• Primary Examiner(s): Martinell, James
• Application Number:
  US12/823,092
• Publication Number:
  US 20100330633A1
• Time in Patent Office: 1,132 Days
• Field of Search: None
• US Class Current: 435/160
• CPC Class Codes: C12M 21/12 for producing fuels or solvents C12M21/04 takes precedence; liquid carbonaceous fuels C10L1/00, solid fuels C10L5/00
• CPC Class Codes: C12M 23/58 Reaction vessels connected in series or in parallel combinations of bioreactors with other apparatus, C12M43/00
• CPC Class Codes: C12M 25/18 Fixed or packed bed
• CPC Class Codes: C12M 25/20 Fluidized bed in chemical or physical processes B01J8/18
• CPC Class Codes: C12M 43/02 Bioreactors or fermenters combined with devices for liquid fuel extraction; Biorefineries
• CPC Class Codes: C12M 45/06 by chemical means or hydrolysis
• CPC Class Codes: C12M 47/10 Separation or concentration of fermentation products bioreactors combined with means for distillation or extraction of liquid fuel C12M43/02
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: C12P 7/16 Butanols
• CPC Class Codes: C12P 7/28 Acetone-containing products
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02P 20/582 Recycling of unreacted starting or intermediate materials

Abstract

Processes and systems for production of bioproducts such as biofuels are provided. The bioproduct production processes and systems utilize pretreatment of a carbohydrate-containing feedstock to produce soluble sugar molecules and continuous conversion of the pretreated feedstock to a bioproduct by an immobilized fermenting microorganism.

Claims

1. A process for producing a bioproduct, comprising:

(i) hydrolyzing a carbohydrate-containing feedstock upstream from a bioreactor that comprises a microbial growth medium and a microorganism that is immobilized on a support in the bioreactor, wherein the carbohydrate-containing feedstock is deconstructed prior to hydrolysis; and

(ii) feeding a resulting hydrolyzed feedstock to the bioreactor,

wherein the hydrolyzed feedstock comprises carbohydrate molecules that serve as a carbon source for growth of the microorganism,

wherein the microorganism ferments the hydrolyzed feedstock to produce the bioproduct, and

wherein the hydrolysis of the feedstock, feeding of the hydrolyzed feedstock to the bioreactor, and production of the bioproduct are continuous.

2. A process according to claim 1, wherein the hydrolyzed feedstock is fed continuously into multiple bioreactors arranged in parallel and/or in series, wherein the bioproduct is produced in each of the multiple bioreactors, wherein the fermentation occurs continuously in the multiple bioreactors, and wherein the multiple bioreactors comprise the same or different microorganism(s).

3. A process according to claim 1, wherein said feedstock is a lignocellulosic material.

4. A process according to claim 3, wherein said lignocellulosic material is pretreated to remove extractives.

5. A process according to claim 4, wherein the pretreatment to remove extractives comprises compression, water extraction, solvent extraction, alkaline extraction, enzymatic treatment, fungal treatment, oxygen treatment, or air drying, wherein the pretreatment occurs prior to or in conjunction with deconstruction.

6. A process according to claim 3, wherein said hydrolysis comprises treatment with nitric acid.

7. A process according to claim 6, wherein said nitric acid hydrolysis comprises a first stage and a second stage, and wherein the second stage hydrolysis is performed at a higher temperature than the first stage.

8. A process according to claim 7, wherein the first and second stage hydrolysates are combined prior to introduction into the bioreactor.

9. A process according to claim 7, wherein the first and second stage hydrolysates are introduced as separate hydrolyzed feedstock streams into separate bioreactors, wherein the first stage hydrolysate is introduced into a first bioreactor and the second stage hydrolysate is introduced into a second bioreactor, wherein the first and second bioreactors comprise the same or different microorganism(s).

10. A process according to claim 9, wherein the first bioreactor comprises a first microorganism and the second bioreactor comprises a second microorganism, wherein the first and second microorganisms are different, and wherein the first microorganism is optimized for growth and/or desired product production on the first stage hydrolysate and the second microorganism is optimized for growth and/or desired product production on the second stage hydrolysate.

11. A process according to claim 7, wherein conditions in the first stage are chosen to achieve hydrolysis of at least about 70% of the hemicellulose in the feedstock, and wherein the conditions in the second stage are chosen to achieve hydrolysis of at least about 40% of the cellulose in the feedstock.

12. A process according to claim 7, wherein flash steam is generated in the second stage hydrolysis, and wherein the flash steam is used to deconstruct the feedstock prior to hydrolysis and/or to provide energy for the first stage hydrolysis.

13. A process according to claim 7, wherein flash steam is generated in the second stage hydrolysis, wherein the flash steam is recompressed, and wherein the recompressed steam is used to provide energy for the first stage hydrolysis and/or a downstream distillation process for product purification.

14. A process according to claim 7, wherein flash steam is generated in the second stage hydrolysis, wherein the flash steam is used to provide energy for a third stage hydrolysis, wherein a temperature of the third stage hydrolysis is lower than a temperature of the second stage hydrolysis, and wherein the lower temperature permits hydrolysis of remaining oligomeric sugar molecules with less degradation than hydrolysis performed at a higher temperature.

15. A process according to claim 3, wherein hydrolysis of the feedstock produces a lignin-containing residue, and wherein the lignin-containing residue is used as an energy source for said process and/or for electricity generation.

16. A process according to claim 3, wherein the lignocellulosic material is wood selected from softwood, hardwood, or a combination thereof.

17. A process according to claim 16, wherein the lignocellulosic material comprises wood chips, sawdust, saw mill residue, or a combination thereof.

18. A process according to claim 3, wherein the lignocelluosic material is from a feedstock source that has been subjected to a disease or infestation.

19. A process according to claim 3, wherein the lignocellulosic material comprises bagasse or straw.

20. A process according to claim 1, wherein said hydrolysis of the feedstock comprises treatment with one or more acid, enzymatic hydrolysis, or autohydrolysis with acetic acid released by the feedstock.

21. A process according to claim 20, wherein the acid comprises one or more acids selected from the group consisting of nitric acid, formic acid, acetic acid, phosphoric acid, hydrochloric acid, and sulfuric acid.

22. A process according to claim 1, wherein hydrolysis comprises treatment with one or more acid, wherein flash steam is generated during the hydrolysis process, and wherein said flash steam is used to provide energy for one or more processes selected from deconstruction of feedstock, further hydrolysis of feedstock, purification of the bioproduct, and recovery of other products of interest.

23. A process according to claim 1, wherein the hydrolyzed feedstock is conditioned to remove inhibitors of microbial growth and/or bioproduct production prior to introduction of the hydrolyzed feedstock into the bioreactor.

24. A process according to claim 1, wherein the microorganism is a Clostridium strain.

25. A process according to claim 24, wherein the Clostridium strain is derived from a species selected from the group consisting of Clostridium saccharobutylicum, Clostridium saccharoperbutylacetonicum, Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium puniceum, Clostridium aurantibutyricum, Clostridium tetanomorphum, Clostridium thermosaccharolyticum, Clostridium butyricum, Clostridium cellulolyticum, Clostridium phytofermentans, Clostridium thermohydrosulfuricum, Clostridium thermobutyricum, Clostridium thermocellum, and Clostridium pasteurianum.

26. A process according to claim 24, wherein the Clostridium strain is an environmental isolate or is derived from an environmental isolate.

27. A process according to claim 1, wherein the immobilized microorganism comprises a biofilm.

28. A process according to claim 1, wherein the bioreactor comprises a packed bed, an expanded bed, or a fluidized bed.

29. A process according to claim 1, wherein the bioproduct comprises a biofuel.

30. A process according to claim 29, wherein the biofuel comprises butanol.

31. A process according to claim 1, wherein bioproduct-containing effluent is continuously withdrawn from the bioreactor, and wherein the process further comprises recovery of the bioproduct from the effluent.

32. A process according to claim 31, wherein the bioproduct comprises butanol, and wherein the recovery comprises distillation to separate butanol from other components of the fermentation medium.

33. A process according to claim 32, wherein butyric acid is recovered in the distillation, and wherein the butyric acid is added to the fermentation medium in the bioreactor, wherein the microorganism converts the butyric acid to butanol.

34. A process according to claim 32, wherein flash steam is generated during hydrolysis of the feedstock and the flash steam provides energy for the distillation.

35. A process according to claim 1, wherein the carbohydrate-containing feedstock is a cellulosic material.

36. A process according to claim 35, wherein the carbohydrate-containing feedstock comprises cellulose and hemicellulose.

37. A process according to claim 1, wherein the deconstruction comprises one or more process selected from presteaming, mechanical grinding, and mechanical explosion.

38. A process according to claim 1, wherein the deconstruction occurs due to one or more natural or intentional causes comprising drought, infestation, fire, or herbicide.

39. A process according to claim 1, wherein the support material in the bioreactor comprises bone char, polypropylene, steel, diatomaceous earth, zeolite, ceramic, engineered thermal plastic, clay brick, concrete, lava rock, wood chips, polyester fiber, glass beads, Teflon, polyetheretherketone, or polyethylene.

40. A process according to claim 1, wherein the bioreactor comprises a packed bed, an expanded bed, or a fluidized bed.