Fluorescent detector systems for the detection of chemical perturbations in sterile storage devices

• US 8,497,134 B2
• Filed: 04/23/2007
• Issued: 07/30/2013
• Est. Priority Date: 08/19/2004
• Status: Active Grant

• Current Assignee: Blood Cell Storage, Inc.
• Original Assignee: Blood Cell Storage, Inc.
• Inventors: Reed, Michael W., Geelhood, Steven J., Harris, Paul C., Barker, Lynn M., Hargrave, Perry, Nanassy, Oliver
• Primary Examiner(s): BASS, DIRK R
• Application Number:
  US11/789,431
• Publication Number:
  US 20070251337A1
• Time in Patent Office: 2,290 Days
• Field of Search: None
• US Class Current: 436/163
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/22 Testing for sterility conditions
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6434 Optrodes
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 2201/0826 Fibre array at source, distributing
• CPC Class Codes: G01N 2201/084 Fibres for remote transmission
• CPC Class Codes: G01N 2400/00 Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
• CPC Class Codes: G01N 33/492 Determining multiple analytes
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

System and method for detecting and measuring chemical perturbations in a sample. The system and method are useful for non-invasive pH monitoring of blood or blood products sealed in storage bags.

Claims

1. A method for monitoring a parameter of a sample, comprising:

(a) irradiating a fluorescent seminaphthofluorescein species having a first emission intensity at a first emission wavelength and a second emission intensity at a second emission wavelength, each emission intensity dependent upon a parameter, which excitation light emanating from a probe physically isolated from the fluorescent species to provide first and second emission intensities, wherein the fluorescent species is in liquid communication with a sample contained in a vessel;

(b) measuring the first and second emission intensities to determine a first parameter reading of the sample; and

(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine a second parameter reading of the sample.

2. The method of claim 1, wherein step (c) is repeated to provide multiple parameter readings to monitor the parameter of the sample over time.

3. The method of claim 1, wherein the parameter is pH.

4. The method of claim 1, wherein the parameter is CO₂.

5. The method of claim 1 further comprising electronically writing parameter readings to the vessel containing the sample.

9. The method of claim 1, wherein the pre-determined time is from about 1 minute to about 1 day.

10. The method of claim 1, wherein the pre-determined time is from about 1 hour to about 12 hours.

11. The method of claim 2, further comprising obtaining a sample profile from the multiple readings.

12. The method of claim 11, further comprising determining sample quality from the profile.

14. The method of claim 1, wherein the fluorescent species is a seminaphthofluorescein compound selected from the group consisting of 5′

(and 6′

)-carboxy-3,10-dihydroxy-spiro[7H-benzo[c]xanthene-7,1′

(3′

H)-isobenzofuran]-3′

-one and 2-(2-chloro-3-hydroxy-9-carboxyethyl-10-oxo-10H-benzo[c]xanthen-7-yl)benzoic acid.

15. The method of claim 1, wherein the fluorescent species is a conjugate of a fluorescent species and a macromolecule.

16. The method of claim 15, wherein the macromolecule is an albumin.

17. The method of claim 1, wherein the fluorescent species is a seminaphthofluorescein/human serum albumin conjugate.

18. The method of claim 1, wherein the sample comprises blood or a blood product.

19. The method of claim 1, wherein the vessel is a sealed vessel.

6. A method for monitoring the pH of a sample, comprising:

(a) irradiating a fluorescent seminaphthofluorescein species in liquid communication with a sample contained in a vessel with excitation light emanating from a probe physically isolated from the fluorescent species, wherein the excitation light has a wavelength sufficient to effect fluorescent emission from the fluorescent species, wherein the fluorescent species exhibits a first emission intensity at a first emission wavelength and a second emission intensity at a second wavelength, the ratio of the first and second emission intensities being dependent upon pH, wherein the first and second emission wavelengths are not the same;

(b) measuring the first and second emission intensities to determine a first parameter reading of the sample; and

(c) repeating step (a) after a pre-determined time and measuring the first and second emission intensities to determine a second parameter reading of the sample.

7. The method of claim 6, wherein step (c) is repeated to provide multiple pH readings to monitor the pH of the sample over time.

8. The method of claim 6 further comprising electronically writing pH readings to the vessel containing the sample.

13. The method of claim 6, wherein the probe is physically isolated from the fluorescent species by a window transparent to the excitation light and the fluorescent emission.