Small volume and fast acting optical analyte sensor
First Claim
1. A sensor for receiving, storing, and chemically reacting with fluid samples from a user for purposes of detecting and quantifying the amount of analytes in such samples by optical means using an optical measurement instrument, such a sensor comprising:
- a base support member, such a base support member providing support for said sensor and having an aperture that allows a portion of a reaction membrane to be exposed to said optical measurement instrument;
said reaction membrane having a surface which is in physical contact with said base support member and covers all of said aperture, said reaction membrane comprising at least one reagent for reacting with at least one compositional component of said analytes and requiring no redox mediators for reaction;
a storage chamber comprising an open-ended capillary passage for conducting said sample from an incision laterally across the width of said storage chamber and simultaneously permitting said sample to saturate said reaction membrane without an additional sorbent material, wherein;
said storage chamber is attached directly to said base support member and said open-ended capillary passage is positioned directly over said reaction membrane;
the open ends of said capillary passage extends to the lateral edges of said base support member and permits saturation of said reaction membrane without an additional sorbent material;
the ventral side of said capillary passage is exposed entirely to said reaction membrane and the width of said capillary passage is no greater than the diameter of said aperture;
said capillary passage is configured to align said sample over an area of said reaction membrane in vertical alignment with said aperture; and
said capillary passage is sized to contain a volume of less than about 1 μ
L of said sample and where the open ends of said capillary passage prevents uncontrolled diffusion of said sample into said storage chamber; and
whereby said sensor is used to measure the amount of analytes in such fluid samples without the use of electrodes on said sensor.
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Accused Products
Abstract
A fast acting sensor designed to accommodate an aqueous analyte-containing sample having a volume of less than one microliter and that can be used to quantify the amount and concentration of such analyte in the sample through light reflectance or fiber-optic light reflectance. The sensor includes a storage chamber, a capillary passage, and a reaction membrane. The storage chamber acts to collect the sample and to secure such sample while it undergoes detection. The capillary passage acts to direct the sample over the reaction membrane and controls the diffusion of the sample in the storage chamber. The reaction membrane contains all of the chemicals and enzymes needed to cause a color-change reaction when contacted with the sample. The amount of analyte can be determined by light reflectance intensity with an optical measurement instrument.
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Citations
8 Claims
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1. A sensor for receiving, storing, and chemically reacting with fluid samples from a user for purposes of detecting and quantifying the amount of analytes in such samples by optical means using an optical measurement instrument, such a sensor comprising:
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a base support member, such a base support member providing support for said sensor and having an aperture that allows a portion of a reaction membrane to be exposed to said optical measurement instrument; said reaction membrane having a surface which is in physical contact with said base support member and covers all of said aperture, said reaction membrane comprising at least one reagent for reacting with at least one compositional component of said analytes and requiring no redox mediators for reaction; a storage chamber comprising an open-ended capillary passage for conducting said sample from an incision laterally across the width of said storage chamber and simultaneously permitting said sample to saturate said reaction membrane without an additional sorbent material, wherein; said storage chamber is attached directly to said base support member and said open-ended capillary passage is positioned directly over said reaction membrane; the open ends of said capillary passage extends to the lateral edges of said base support member and permits saturation of said reaction membrane without an additional sorbent material; the ventral side of said capillary passage is exposed entirely to said reaction membrane and the width of said capillary passage is no greater than the diameter of said aperture; said capillary passage is configured to align said sample over an area of said reaction membrane in vertical alignment with said aperture; and said capillary passage is sized to contain a volume of less than about 1 μ
L of said sample and where the open ends of said capillary passage prevents uncontrolled diffusion of said sample into said storage chamber; andwhereby said sensor is used to measure the amount of analytes in such fluid samples without the use of electrodes on said sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification