Synthetic blocking reagents

• US 8,501,199 B2
• Filed: 06/10/2010
• Issued: 08/06/2013
• Est. Priority Date: 06/10/2009
• Status: Active Grant

• Current Assignee: Borstel Forschungszentrum
• Original Assignee: Borstel Forschungszentrum
• Inventors: Fujimoto, Naho, Bade, Steffen, Ramaker, Katrin, Frey, Andreas, Rckendorf, Niels
• Primary Examiner(s): Haq, Shafiqul
• Application Number:
  US12/797,998
• Publication Number:
  US 20100317048A1
• Time in Patent Office: 1,153 Days
• Field of Search: None
• US Class Current: 424/278.1
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention concerns novel synthetic blocking reagents for the reduction of non-specific bindings in solid phase assays that rely on biological and specific recognition, e.g., in enzyme-linked immunosorbent assays (ELISAs). The invention provides the use of compounds as blocking reagents as well as kits comprising these compounds. The compounds comprise one or more poly(ethylene glycol) moieties, one or more alkyl- or aminoalkyl groups and another unit bridging the aforementioned groups, wherein the compound comprises at least one amino group.

Claims

1. A blocking reagent comprising a compound of Formula II:

2. The compound of Formula II of claim 1, wherein

PEG is a linear poly(ethylene glycol) having an average molecular weight of 300-3,500 Da, preferably, 400-2,500 Da, and

R¹ is selected form the group consisting of —

H, —

CH₃, —

CH₂CH₃, —

CH₂CH₂CH₃ and —

CH(CH₃)₂ and R² is —

H.

3. The compound of Formula II of claim 1, wherein R¹ is —

CH₃, R² is —

H, PEG is a linear poly(ethylene glycol) with an average molecular weight of 400-700 Da and Alkyl is —

(CH₂)₁₂—

or —

(CH₂)₂₀—

.