Soybean transgenic event MON 87708 and methods of use thereof

• US 8,501,407 B2
• Filed: 08/26/2010
• Issued: 08/06/2013
• Est. Priority Date: 09/17/2009
• Status: Expired

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Brinker, Ronald J., Burns, Wen C., Feng, Paul C. C., Gupta, Anju, Hoi, Sio-Wai, Malven, Marianne, Wu, Kunsheng
• Primary Examiner(s): Horlick, Kenneth R.
• Assistant Examiner(s): OYEYEMI, OLAYINKA A
• Application Number:
  US12/868,989
• Publication Number:
  US 20110067134A1
• Time in Patent Office: 1,076 Days
• Field of Search: 800/260, 800/300, 800/314, 800/312, 435/6.11, 435/6.12, 435/410, 435/415, 435/418, 435/419, 504/127, 504/206, 504/324, 536/23.1, 536/23.6, 536/24.3, 426/634, 426/630, 426/592, 111/100, 56/1
• US Class Current: 435/6.11
• CPC Class Codes: A23D 9/00 Other edible oils or fats, e.g. shortenings, cooking oils
• CPC Class Codes: A23L 11/03 Soya beans, e.g. full-fat soya bean flakes or grits
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Y 114/00 Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The invention provides a transgenic soybean event MON 87708 plant and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87708. The invention also provides polynucleotides specific for event MON 87708 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87708. The invention also provides methods related to event MON 87708.

Claims

1. A recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:

1-8, and full complements thereof.

2. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

1.

3. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

2.

4. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

3.

5. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

4.

6. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

5.

7. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

6.

8. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

7.

9. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

8.

10. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

1 and SEQ ID NO;

2.

11. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

3 and SEQ ID NO;

4.

12. The recombinant DNA molecule of claim 1, comprising the nucleotide sequence of SEQ ID NO:

7 and SEQ ID NO;

8.

13. The recombinant DNA molecule of claim 1, wherein said recombinant DNA molecule is from transgenic soybean event MON 87708, a representative sample of seed comprising said event having been deposited as ATCC PTA-9670.

14. The recombinant DNA molecule of claim 1, wherein said recombinant DNA molecule is in a soybean plant, soybean plant cell, soybean seed, soybean plant part, or commodity product.

15. A DNA primer or probe comprising a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

6, or full complements thereof, wherein said DNA primer or probe is diagnostic for event MON 87708 and wherein said DNA primer or probe hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-8 and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NO;

1-8.

17. A method of detecting the presence of a DNA molecule from soybean event MON 87708 in a sample, said method comprising:

a) contacting said sample with the DNA probe of claim 15;

b) subjecting said sample and said DNA probe to stringent hybridization conditions;

and c) detecting hybridization of said DNA probe to a DNA molecule in said sample, wherein the hybridization of said DNA probe to said DNA molecule indicates the presence of a DNA molecule from soybean event MON 87708 in said sample.

16. A pair of DNA molecules comprising of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first and second DNA molecules each comprise a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

6, or a full complement thereof, to function as DNA primers that when used together in an amplification reaction with DNA from event MON 87708 produce an amplicon diagnostic for soybean event MON 87708 DNA in a sample.

18. A method of detecting the presence of a DNA molecule from soybean event MON 87708 in a sample, said method comprising:

a) contacting said sample with the pair of DNA molecules of claim 16;

b) performing an amplification reaction sufficient to produce a DNA amplicon comprising a sequence selected from the group consisting of SEQ ID NO;

1-8, and full complements thereof; and

c) detecting the presence of said DNA amplicon in said reaction, wherein the presence of said DNA amplicon in said reaction indicates the presence of a DNA molecule from soybean event MON 87708 in said sample.

19. A method of determining the zygosity of a soybean plant or seed comprising event MON 87708 comprising:

a) contacting a sample comprising soybean DNA with a primer set and a probe set capable of producing a first amplicon and a second amplicon;

b) performing a nucleic acid amplification reaction with said sample, primer set, and a probe set;

c) detecting in said nucleic acid amplification reaction said first amplicon that is diagnostic for event MON 87708 and said second amplicon that diagnostic for native soybean genomic DNA not comprising event MON 87708; and

d) analyzing for the presence and/or absence of said first amplicon and said second amplicon in said nucleic acid amplification reaction, wherein the presence of both amplicons indicates said sample is heterozygous for event MON 87708 and the presence of only said first amplicon indicates said sample is homozygous for event MON 87708.

20. The method of claim 19, wherein the primer set comprises SEQ ID NO:

12, SEQ ID NO;

13 and SEQ ID NO;

14.

21. The method of claim 19, wherein the probe set comprises SEQ ID NO:

15 and SEQ ID NO;

16.

22. A DNA detection kit comprising a pair of DNA primers or at least one DNA probe, wherein a) the pair of DNA primers comprises at least one DNA molecule comprising a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

6, or full complements thereof, wherein the pair of DNA primers is capable of producing an amplicon that is diagnostic for event MON 87708; and

b) the at least one DNA probe is diagnostic for event MON 87708.