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Homologous recombination-based DNA cloning compositions

  • US 8,501,454 B2
  • Filed: 01/31/2012
  • Issued: 08/06/2013
  • Est. Priority Date: 09/10/2008
  • Status: Active Grant
First Claim
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1. A kit for use in cloning a donor DNA molecule into an acceptor vector at a predetermined location, the kit consisting essentially of:

  • (1) a composition comprising;

    a) an enzyme cocktail consisting essentially of an exonuclease having only 3′

    - or 5′

    -exonucleolytic single-strand degradation activity, and a single-stranded DNA binding protein; and

    b) a reaction buffer;

    (2) instructions on using the composition in the cloning, and(3) a competent cell for use in the cloning.

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