Electrochemical detection system

• US 8,506,908 B2
• Filed: 03/10/2008
• Issued: 08/13/2013
• Est. Priority Date: 03/09/2007
• Status: Active Grant

• Current Assignee: Vantix Holdings Limited
• Original Assignee: Vantix Holdings Limited
• Inventors: Benn, Jim, Carvalho, Bruce, Gover, Andy, Morris, Roger
• Primary Examiner(s): Sines, Brian J.
• Assistant Examiner(s): Wecker, Jennifer
• Application Number:
  US12/045,611
• Publication Number:
  US 20080217246A1
• Time in Patent Office: 1,982 Days
• Field of Search: 204/403.01, 204/403.14, 204/403.02, 204/403.03, 204/400, 422/68.1, 422/82.01, 422/82.03, 422/554, 422/401
• US Class Current: 422/554
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 2035/00881 network configurations
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

An electrochemical detection system having a disposable cartridge capable of performing a plurality of assay protocols is disclosed. The cartridge includes a blister pack for the long-term storage and controlled release of multiple reagents. The blister pack is bonded to and operatively associated with a fluidic backbone for providing the fluid pathways, storage capacity, and fluid control functions for performing multiple assay protocols. The cartridge further includes a plurality of sensors having a multiple electrode arrangement in operative association with a respective flow cell defined by the fluidic backbone. After the user has transferred a sample into the cartridge and engaged the cartridge to the reader, the reader operatively interfaces with the cartridge such that different assay protocols may be simultaneously performed in isolation from one another inside the cartridge. The reader may be one of many readers that operatively communicate data to a remote server for processing.

Claims

1. A cartridge for performing a plurality of assay protocols comprising:

a blister pack including a plurality of blister domes for storing a respective reagent;

the blister pack having a deformable upper portion and a frangible lower portion for the controlled release of the respective reagent when a mechanical force is applied to one or more of the plurality of blister domes;

a fluidic backbone engaged to the blister pack, the fluidic backbone including a plurality of channels in selective fluid flow communication with a plurality of flow cells through an arrangement of valves such that each of the plurality of flow cells is isolated from the other flow cells during the performance of a plurality of assay protocols, the fluidic backbone defining at least one puncture site base, wherein the at least one puncture site base comprises a raised inner rim and a raised outer rim and defines an outlet and a well in fluid communication with at least one of the plurality of channels;

a plurality of sensors operatively associated with a respective flow cell for detecting a reaction during the performance of a plurality of assays;

wherein each of the plurality of sensors comprises at least one working electrode and a reference electrode supported by a backing;

wherein the deformable upper portion and the frangible lower portion define a finger channel in fluid flow communication between one of the plurality of blister domes and at least one puncture valve site; and

wherein the blister pack is engaged to the raised inner rim and the raised outer rim so that the at least one puncture site base is positioned immediately adjacent to the at least one puncture valve site.

2. The cartridge of claim 1, further including a flexible adhesive film engaged between the blister pack and the fluidic backbone.

3. The cartridge of claim 2, wherein a sample reservoir is formed between the flexible adhesive film and the fluidic backbone.

4. The cartridge of claim 3, wherein the sample reservoir deforms the flexible adhesive film when a liquid fills the space between the fluidic backbone and the flexible adhesive film.

5. The cartridge of claim 1, wherein the arrangement of valves includes a plurality of valve sites defined by the blister pack that correspond to a plurality of valves in communication with a respective channel defined by the fluidic backbone for collectively preventing or permitting fluid flow through the respective channel, wherein each of the plurality of valves defines an inlet in selective fluid flow communication with an outlet through a well, wherein each of the plurality of valve sites includes a deformable material adapted to engage the valve to prevent fluid flow communication between the inlet and the outlet and disengage from the valve to permit fluid flow communication between the inlet and the outlet.

6. The cartridge of claim 5, wherein the cartridge is adapted to operatively engage a reader such that the reader mechanically actuates one or more of the plurality of valve sites to prevent or permit fluid flow communication by engaging or disengaging the one or more of the valve sites relative to a respective one of the plurality of valves.

7. The cartridge of claim 1, wherein the cartridge is adapted to operatively engage a reader such that the reader mechanically actuates one or more of the plurality of blister domes such that the deformable upper portion becomes deformed while the frangible lower portion breaks apart.

8. The cartridge of claim 7, wherein each of the plurality of blister domes contains a fluid, and each of the plurality of blister domes is in fluid flow communication with a puncture valve site that corresponds to a puncture site base for establishing fluid flow communication with a channel when the puncture site base is mechanically actuated by the reader.

9. The cartridge of claim 8, wherein fluid from the plurality of blister domes is released into the respective channel by mechanically actuating the puncture valve site to establish fluid flow communication with the respective channel, and then mechanically actuating the plurality of blister domes such that liquid is released from the plurality of blister domes and through a respective puncture valve site, wherein mechanical actuation of the plurality of blister domes and the puncture valve sites causes the deformable upper portion to become deformed and the frangible lower portion to break apart in order to establish fluid flow communication between the plurality of blister domes and one of the plurality of channels upon mechanical actuation by the reader.

10. The cartridge of claim 1, wherein each of the plurality of sensors includes an arrangement of four electrodes that are operatively associated with a respective one of the plurality of flow cells for detecting an electrochemical reaction.

11. The cartridge of claim 10, wherein the arrangement of four electrodes includes three working electrodes and a reference electrode.

12. The cartridge of claim 11, wherein at least one of the working electrodes is a working calibration electrode for providing a means of calibrating each of the plurality of sensors.

13. The cartridge of claim 1, further including a bottom lid that is engaged to the fluidic backbone for defining the plurality of channels.

14. The cartridge of claim 1, wherein the fluid flow is initiated by mechanically engaging the puncture valve site against the puncture site base to establish fluid flow communication between one of the plurality of blister domes and the puncture site base, and then mechanically actuating the one of the plurality of blister domes to permit the release of liquid through the outlet of the puncture site base.

15. The cartridge of claim 1, wherein a fluidic pathway is defined between the deformable upper portion and the frangible lower portion such that the mechanical actuation of the blister pack causes the deformable upper portion to deform and the frangible lower portion to break apart.

16. A cartridge for performing a plurality of assay protocols comprising:

a blister pack including a plurality of blister domes for storing a respective reagent;

the blister pack having a deformable upper portion and a frangible lower portion for the controlled release of the respective reagent when a mechanical force is applied to one or more of the plurality of blister domes;

a fluidic backbone engaged to the blister pack, the fluidic backbone including a plurality of channels in selective fluid flow communication with a plurality of flow cells through an arrangement of valves such that each of the plurality of flow cells is isolated from the other flow cells during the performance of a plurality of assay protocols;

a plurality of sensors operatively associated with a respective flow cell for detecting a reaction during the performance of a plurality of assays;

wherein the cartridge is adapted to operatively engage a reader capable of mechanically actuating the cartridge from only one side of the cartridge; and

wherein the reader mechanically actuates the cartridge using a plurality of actuating pistons or rollers.