Dual inlet microchannel device and method for using same

• US 8,512,649 B2
• Filed: 11/16/2012
• Issued: 08/20/2013
• Est. Priority Date: 04/07/2006
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Bergman, Richard, Lewis, Mark Alan, Li, Cheng-Chung, Miller, William James
• Primary Examiner(s): Jarrett, Lore
• Application Number:
  US13/679,300
• Publication Number:
  US 20130071935A1
• Time in Patent Office: 277 Days
• Field of Search: 422/503
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/565 Seals in general F16L
• CPC Class Codes: G01N 33/557 using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction

Abstract

A dual inlet microchannel device and a method for using the device to perform a flow-through kinetic assay are described. A microplate having an array of the dual inlet microchannel devices and in particular their specially configured flow chambers is also described. Several embodiments of the dual inlet microchannel devices and specially configured flow chambers are also described.

Claims

1. A method for performing a kinetic assay, the method comprising the steps of:

attaching a fluid handling system to a device, where the fluid handling system includes a first fluid delivery tip, a second fluid delivery tip, and a fluid removal tip all of which are connected to the device, wherein the device has a body including;

a first fluid inlet connected to the first fluid delivery tip;

a second fluid inlet connected to the second fluid delivery tip; and

a fluid outlet connected to the fluid removal tip; and

wherein the body has a flow chamber formed therein, wherein the flow chamber including;

a first flow restrictor associated with the first fluid inlet;

a second flow restrictor associated with the second fluid inlet;

a central portion having one corner of one end associated with the first flow restrictor and another corner of the one end associated with the second flow restrictor;

the central portion having a sensing surface on which there is immobilized a target; and

an outlet portion associated with the fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion;

flowing a buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while an inspection system obtains a baseline measurement;

stopping the flow of the buffer solution;

flowing an analyte solution through the second fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains an association measurement;

stopping the flow of the analyte solution; and

flowing the buffer solution through the first fluid delivery tip and into and through the flow chamber and then out the fluid removal tip while the inspection system obtains a disassociation measurement.

2. The method of claim 1, wherein the first flow restrictor is a first porous material and the second flow restrictor is a second porous material.

3. The method of claim 1, wherein the first flow restrictor is a first weir and the second flow restrictor is a second weir.

4. The method of claim 1, wherein the flow chamber further includes:

a first inlet portion located between the first fluid inlet and the first flow restrictor; and

a second inlet portion located between the second fluid inlet and the second flow restrictor.

5. The method of claim 4, wherein the first flow restrictor is a first flow restrictive neck and the second flow restrictor is a second flow restrictive neck.

6. The method of claim 5, wherein the first flow restrictive neck is sized to at least minimize the buffer solution from flowing into the second inlet portion and up the second fluid inlet.

7. The method of claim 5, wherein the second flow restrictive neck is sized to at least minimize the analyte solution from flowing into the first inlet portion and up the first fluid inlet.

8. The method of claim 5, wherein the central portion further includes a third flow restrictive neck which is in fluid communication with the first flow restrictive neck and the second flow restrictive neck.

9. The method of claim 8, wherein the third flow restrictive neck is sized to assure the buffer solution or analyte solution flows directly over the sensing surface.

10. The method of claim 1, wherein the inspection system comprises at least one of:

a grating-based inspection system;

a surface plasmon resonance inspection system;

a fluorescence inspection system;

an acousto-optic inspection system;

a capacitive inspection system;

a visual inspection system;

or

a combination thereof.