Biological material and methods and solutions for preservation thereof

• US 8,512,941 B2
• Filed: 03/29/2011
• Issued: 08/20/2013
• Est. Priority Date: 02/02/2004
• Status: Expired due to Fees

• Current Assignee: Core Dynamics Limited
• Original Assignee: Core Dynamics Limited
• Inventors: Natan, Yehudit
• Primary Examiner(s): Russel, Jeffrey E
• Application Number:
  US13/074,843
• Publication Number:
  US 20110177488A1
• Time in Patent Office: 875 Days
• Field of Search: None
• US Class Current: 435/2
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0221 Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
• CPC Class Codes: A61L 2/0052 Visible light
• CPC Class Codes: A61L 2202/22 Blood or products thereof

Abstract

Provided is a preservation solution for preserving biological material at low temperature including one or more polyphenols and a method for preservation of biological material, the method includes adding the preservation solution to biological material, cooling the biological material and storing it under appropriate storing conditions. The present method may be used for hypothermic preservation or for cryopreservation, including freezing and lyophilization, and may be used with any biological material, including cells selected from RBC, WBC, MNC, UCB MNC and bacteria. In the case of RBC, also disclosed is a method for its freezing such that upon thawing, the material has less than 2% free hemoglobin.

Claims

1. A method for preserving red blood cells (RBC), comprising:

adding a preservation solution to the RBC, the preservation solution comprising one or more polyphenols;

freeze drying the RBC to produce frozen RBC; and

storing the frozen RBC under appropriate storing conditions,

wherein, post preservation, the RBC comprise free hemoglobin levels below 10 percent.

2. The method of claim 1, wherein the polyphenols comprise one or more catechins.

3. The method of claim 2, wherein at least one of the one or more catechins is epigallocatechin gallate (EGCG).

4. The method of claim 1, wherein the polyphenols are derived from green tea extract (GTE).

5. The method of claim 1, wherein the preservation solution does not comprise a significant amount of glycerol.

6. The method of claim 1, wherein the preservation solution does not comprise a significant amount of DMSO.

7. The method of claim 1, wherein the preservation solution comprises a macromolecule.

8. The method of claim 1, wherein the preservation is cryopreservation, the preservation solution is a cryopreservation solution and the freeze drying is to a temperature below 0°

C.

9. The method of claim 1, wherein the one or more polyphenols are present in the preservation solution at a concentration of from about 50 μ

M to about 4 mM.