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Vectors with modified initiation codon for the translation of AAV-Rep78 useful for production of AAV

  • US 8,512,981 B2
  • Filed: 06/20/2007
  • Issued: 08/20/2013
  • Est. Priority Date: 06/21/2006
  • Status: Active Grant
First Claim
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1. A method for producing a recombinant parvoviral virion in an insect cell, comprising the steps of:

  • (a) culturing an insect cell comprising;

    (i) a first nucleotide sequence comprising;

    (A) a single open reading frame (ORF) encoding parvoviral Rep proteins Rep78 and Rep52; and

    (B) a suboptimal initiation codon selected from the group consisting of ACG, TTG, CTG and GTG, and(ii) not comprising a nucleotide sequence encoding parvoviral Rep proteins other than said first nucleotide sequence,under conditions such that the recombinant parvoviral virion is produced; and

    (b) recovering the recombinant parvoviral virion.

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