Catalytic domains from lysyl oxidase and LOXL2

• US 8,512,990 B2
• Filed: 08/20/2010
• Issued: 08/20/2013
• Est. Priority Date: 08/21/2009
• Status: Active Grant

• Current Assignee: Gilead Biologics, Inc. (Gilead Sciences Inc.)
• Original Assignee: Gilead Biologics, Inc. (Gilead Sciences Inc.)
• Inventors: McCauley, Scott, Smith, Victoria
• Primary Examiner(s): Saidha, Tekchand
• Assistant Examiner(s): Meah, Younus
• Application Number:
  US12/860,625
• Publication Number:
  US 20110076739A1
• Time in Patent Office: 1,096 Days
• Field of Search: None
• US Class Current: 435/189
• CPC Class Codes: C12N 9/0022 with oxygen as acceptor (1.4.3)
• CPC Class Codes: C12Y 104/03013 Protein-lysine 6-oxidase (1.4.3.13), i.e. lysyl-oxidase

Abstract

Disclosed herein are amino acid sequences, and encoding nucleotide sequences, of isolated catalytic domains of the LOX and LOXL2 proteins from human and mouse. Methods for the preparation and use of these isolated catalytic domains are also provided.

Claims

1. An isolated polypeptide, comprising:

a catalytic domain of a human LOXL2 protein, wherein;

the catalytic domain comprises the amino acid sequence selected from the group consisting of;

SEQ ID NO;

3, and SEQ ID NO;

3 without the initial TA residues; and

the polypeptide does not contain sequences from the human LOXL2 protein outside its catalytic domain.

2. The polypeptide of claim 1, wherein the catalytic domain is of a human LOXL2 and comprises the amino acid sequence set forth in SEQ ID NO:

3.

3. The polypeptide of claim 1, further comprising a signal sequence, an epitope tag and a purification tag.

4. The polypeptide of claim 3, wherein the signal sequence is an immunoglobulin kappa signal sequence.

5. The polypeptide of claim 3, wherein the epitope tag is myc tag.

6. The polypeptide of claim 3, wherein the purification tag is a His₆ tag.

7. The polypeptide of claim 2, further comprising a signal sequence, an epitope tag and a purification tag.

8. The polypeptide of claim 7, wherein the signal sequence is an immunoglobulin kappa signal sequence.

9. The polypeptide of claim 7, wherein the epitope tag is myc tag.

10. The polypeptide of claim 7, wherein the purification tag is a His₆ tag.

11. The polypeptide of claim 7, wherein the polypeptide has an amino acid sequence as set forth in SEQ ID NO:

11.