Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells

• US 8,513,009 B2
• Filed: 01/29/2009
• Issued: 08/20/2013
• Est. Priority Date: 01/30/2008
• Status: Active Grant

• Current Assignee: Asterias Biotherapeutics Incorporated (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Geron Corporation
• Inventors: Shogbon, Christopher Bankole, Zhou, Yue, Brandenberger, Ralph
• Primary Examiner(s): Ton, Thaian N
• Application Number:
  US12/362,250
• Publication Number:
  US 20090191626A1
• Time in Patent Office: 1,664 Days
• Field of Search: 435/368, 435/395, 435/402
• US Class Current: 435/368
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0622 Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
• CPC Class Codes: C12N 5/0623 Stem cells

Abstract

Synthetic surfaces suitable for culturing stem cell derived oligodendrocyte progenitor cells contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived oligodendrocyte progenitor cells in chemically defined media.

Claims

1. A culture of human stem cell derived oligodendrocyte progenitor cells, comprising:

an article comprising a polymer material disposed on a surface, wherein the polymer material supports adhesion of the human stem-cell derived oligodendrocyte progenitor cells;

the human stem cell derived oligodendrocyte progenitor cells disposed on the polymer material; and

a culture medium in which the oligodendrocyte progenitor cells are cultured, wherein the polymer material comprises an acrylate selected from;

a homopolymer formed from tetra(ethylene glycol)diacrylate; and

a copolymer selected from;

tetra(ethylene glycol)diacrylate and neopentyl glycol ethoxylate diacrylate,

glycerol dimethacrylate and tetra(ethylene glycol)diacrylate,

glycerol dimethacrylate and tri(ethylene glycol)dimethacrylate,

glycerol dimethacrylate and di(ethylene glycol)dimethacrylate,

glycerol dimethacrylate and tetraethylene glycol dimethacrylate,

glycerol dimethacrylate and 1,6-hexanediol propoxylate diacrylate,

glycerol dimethacrylate and 1,6-hexanediol ethoxylate diacrylate,

tri(ethylene glycol)dimethacrylate and trimethylolpropane triacrylate,

1,4-butanediol dimethacrylate and 1,9 nonanediol diacrylate,

1,6-hexanediol diacrylate and tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

1,6-hexanediol diacrylate and 1,6-hexanediol ethoxylate diacrylate,

1,6-hexanediol diacrylate and neopentyl glycol ethoxylate diacrylate,

neopentyl glycol propoxylate (1PO/OH) diacrylate and neopentyl glycol ethoxylate diacrylate,

di(ethylene glycol)dimethacrylate and neopentyl glycol diacrylate,

di(ethylene glycol)dimethacrylate and

tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate,

tetra(ethylene glycol)dimethacrylate and neopentyl glycol ethoxylate diacrylate,

1,6-hexanediol propoxylate diacrylate and neopentyl glycol ethoxylate diacrylate,

neopentyl glycol diacrylate and tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

neopentyl glycol diacrylate and 1,6-hexanediol ethoxylate diacrylate,

neopentyl glycol diacrylate and poly(propylene glycol)diacrylate,

trimethylolpropane ethoxylate (1 EO/OH) methyl diacrylate and 2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate,

neopentyl glycol ethoxylate diacrylate and di(ethylene glycol)dimethacrylate,

trimethylolpropane triacrylate and 1,4-butanediol dimethacrylate,

trimethylolpropane triacrylate and di(ethylene glycol)dimethacrylate,

trimethylolpropane triacrylate and neopentyl glycol diacrylate,

trimethylolpropane triacrylate and neopentyl glycol dimethacrylate,

2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate and tetra(ethylene glycol)dimethacrylate,

2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate and neopentyl glycol diacrylate, and

poly(propylene glycol)diacrylate and glycerol 1,3-diglycerolate diacrylate.

2. The culture of claim 1, wherein the human stem cell derived oligodendrocyte progenitor cells are human embryonic stem cell derived oligodendrocyte progenitor cells.

6. The culture of claim 1, wherein the acrylate comprises tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate.

3. A method for culturing human stem cell derived

oligodendrocyte progenitor cells, comprising:

depositing a suspension comprising the human stem cell derived oligodendrocyte progenitor cells on a polymer material; and

culturing the deposited human stem cell derived oligodendrocyte progenitor cells in a cell culture medium, wherein the polymer material comprises an acrylate selected from;

a homopolymer formed from tetra(ethylene glycol)diacrylate; and

a copolymer selected from;

tetra(ethylene glycol)diacrylate and neopentyl glycol ethoxylate diacrylate,

glycerol dimethacrylate and tetra(ethylene glycol)diacrylate,

glycerol dimethacrylate and tri(ethylene glycol)dimethacrylate,

glycerol dimethacrylate and di(ethylene glycol)dimethacrylate,

glycerol dimethacrylate and tetraethylene glycol dimethacrylate,

glycerol dimethacrylate and 1,6-hexanediol propoxylate diacrylate,

glycerol dimethacrylate and 1,6-hexanediol ethoxylate diacrylate,

tri(ethylene glycol)dimethacrylate and trimethylolpropane triacrylate,

1,4-butanediol dimethacrylate and 1,9 nonanediol diacrylate,

1,6-hexanediol diacrylate and tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

1,6-hexanediol diacrylate and 1,6-hexanediol ethoxylate diacrylate,

1,6-hexanediol diacrylate and neopentyl glycol ethoxylate diacrylate,

neopentyl glycol propoxylate (1PO/OH) diacrylate and neopentyl glycol ethoxylate diacrylate,

di(ethylene glycol)dimethacrylate and neopentyl glycol diacrylate,

di(ethylene glycol)dimethacrylate and

tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate,

tetra(ethylene glycol)dimethacrylate and neopentyl glycol ethoxylate diacrylate,

1,6-hexanediol propoxylate diacrylate and neopentyl glycol ethoxylate diacrylate,

neopentyl glycol diacrylate and tricyclo[5.2.1.0^2,6]decanedimethanol diacrylate,

neopentyl glycol diacrylate and 1,6-hexanediol ethoxylate diacrylate,

neopentyl glycol diacrylate and poly(propylene glycol)diacrylate,

trimethylolpropane ethoxylate (1 EO/OH) methyl diacrylate and 2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate,

neopentyl glycol ethoxylate diacrylate and di(ethylene glycol)dimethacrylate,

trimethylolpropane triacrylate and 1,4-butanediol dimethacrylate,

trimethylolpropane triacrylate and di(ethylene glycol)dimethacrylate,

trimethylolpropane triacrylate and neopentyl glycol diacrylate,

trimethylolpropane triacrylate and neopentyl glycol dimethacrylate,

2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate and tetra(ethylene glycol)dimethacrylate,

2,2,3,3,4,4,5,5 octafluoro 1,6 hexanediol diacrylate and neopentyl glycol diacrylate, and

poly(propylene glycol)diacrylate and glycerol 1,3-diglycerolate diacrylate;

wherein the polymer material supports adhesion of the human stem cell derived oligodendrocyte progenitor cells.

4. The method of claim 3, wherein the cell culture medium is a chemically defined medium.

5. The method of claim 3, wherein the human stem cell derived oligodendrocyte progenitor cells are human embryonic stem cell derived oligodendrocyte progenitor cells.

7. The method of claim 3, wherein the acrylate comprises tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate.

8. A culture of human stem cell derived oligodendrocyte progenitor cells, comprising:

an article comprising a polymer material disposed on a surface, wherein the polymer material supports adhesion of the human stem cell derived oligodendrocyte progenitor cells;

the human stem cell derived oligodendrocyte progenitor cells disposed on the polymer material; and

a culture medium comprising insulin, transferrin, progesterone, putrescin, selenium, triiodothyroidin and B27 in which the human stem cell derived oligodendrocyte progenitor cells are cultured, wherein the polymer material comprises tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate.

9. A culture of human stem cell derived oligodendrocyte progenitor cells, comprising:

an article comprising a polymer material disposed on a surface, wherein the polymer material supports adhesion of the human stem cell derived oligodendrocyte progenitor cells;

the human stem cell derived oligodendrocyte progenitor cells disposed on the polymer material; and

a culture medium comprising triiodothyroidin and B27 in which the human stem cell derived oligodendrocyte progenitor cells are cultured, wherein the polymer material comprises tetra(ethylene glycol)dimethacrylate and 1,4-butanediol dimethacrylate.