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Method for production of mast cells from stem cells

  • US 8,513,012 B2
  • Filed: 05/04/2009
  • Issued: 08/20/2013
  • Est. Priority Date: 05/02/2008
  • Status: Active Grant
First Claim
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1. A method of preparing human mast cells by culturing human pluripotent cells in vitro comprising the steps of:

  • a) culturing human pluripotent cells under conditions that promote differentiation of the cells into hematopoietic progenitor cells or megakaryocytes, to provide a first cell population comprising hematopoietic precursors or megakaryocytes; and

    b) culturing the first cell population under conditions that promote the differentiation into mast cells, to provide a second cell population comprising mast cells;

    wherein the step (a) culturing step does not employ a co-culture with murine fetal liver-derived stromal cells as a feeder layer;

    wherein the step (b) culturing step comprises culturing the cells in a media comprising stem cell factor;

    further wherein the pluripotent cells are cultured in a media comprising TPO.

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