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Laminin-entactin complex and cell culture article and methods thereof

  • US 8,513,015 B2
  • Filed: 05/19/2011
  • Issued: 08/20/2013
  • Est. Priority Date: 06/11/2010
  • Status: Active Grant
First Claim
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1. A cell culture system comprising:

  • a substrate;

    a coating on the substrate;

    one or more live cells on the coated substrate; and

    an overlay layer atop the live cells, the substrate coating consists of an isolated and purified, laminin, and the overlay layer consists of an isolated and purified, laminin and entactin complex having the laminin and the entactin associated in a mole ratio of 1;

    1, and the live cells are selected from hepatocyctes, primary hepatocytes, mammalian hepatic cell lines, stem cells, or combinations thereof.

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