Laminin-entactin complex and cell culture article and methods thereof
First Claim
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1. A cell culture system comprising:
- a substrate;
a coating on the substrate;
one or more live cells on the coated substrate; and
an overlay layer atop the live cells, the substrate coating consists of an isolated and purified, laminin, and the overlay layer consists of an isolated and purified, laminin and entactin complex having the laminin and the entactin associated in a mole ratio of 1;
1, and the live cells are selected from hepatocyctes, primary hepatocytes, mammalian hepatic cell lines, stem cells, or combinations thereof.
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Abstract
A cell culture system including: a substrate, a substrate coating, one or more live cells, and an overlay source. The substrate coating and overlay can be laminin, and laminin•entactin complex, respectively. Alternatively, the substrate coating and overlay can be laminin•entactin complex, and laminin, respectively. The cell culture system can further include liquid media and a protective cover. A method for making and using the system in cell culture articles and culture methods, as defined herein, is also disclosed.
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Citations
17 Claims
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1. A cell culture system comprising:
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a substrate; a coating on the substrate; one or more live cells on the coated substrate; and an overlay layer atop the live cells, the substrate coating consists of an isolated and purified, laminin, and the overlay layer consists of an isolated and purified, laminin and entactin complex having the laminin and the entactin associated in a mole ratio of 1;
1, and the live cells are selected from hepatocyctes, primary hepatocytes, mammalian hepatic cell lines, stem cells, or combinations thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 12, 13, 15, 16)
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9. A method of making a cell culture article comprising:
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laminin coating a substrate, the laminin coating consisting of an isolated and purified laminin; contacting the laminin coated substrate with live cells to form at least a monolayer of the live cells on the coated laminin substrate; providing an overlay source consisting of an isolated and purified laminin and entactin complex to form an isolated and purified laminin and entactin complex overlay on the live cells on the coated substrate. - View Dependent Claims (10, 11)
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14. A cell culture article comprising:
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a substrate; an isolated and purified, laminin layer on the substrate; a live cell layer on the isolated and purified, laminin layer; and an isolated and purified, laminin and entactin complex overlay layer on the cell layer, and the live cells of the live cell layer are selected from hepatocyctes, primary hepatocytes, mammalian hepatic cell lines, stem cells, or combinations thereof. - View Dependent Claims (17)
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Specification