Planar lipid bilayer array formed by microfluidic technique and method of analysis using planar lipid bilayer

• US 8,513,165 B2
• Filed: 11/26/2008
• Issued: 08/20/2013
• Est. Priority Date: 11/26/2007
• Status: Active Grant

• Current Assignee: University of Tokyo
• Original Assignee: University of Tokyo
• Inventors: Takeuchi, Shoji, Suzuki, Hiroaki, Ota, Sadao, Tan, Wei-Heong
• Primary Examiner(s): Marschel, Ardin
• Assistant Examiner(s): Flinders, Jeremy C
• Application Number:
  US12/744,794
• Publication Number:
  US 20100304980A1
• Time in Patent Office: 1,728 Days
• Field of Search: None
• US Class Current: 506/33
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: C40B 60/00 Apparatus specially adapted for use in combinatorial chemistry or with libraries
• CPC Class Codes: G01N 2405/04 Phospholipids, i.e. phosphoglycerides
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743

Abstract

There is provided a planar lipid bilayer array formed by microfluidic technique and a method of analysis using the planar lipid bilayers, providing the advantages such as portability, decreased analysis time, a smaller amount of required reagents, and parallel automation with high reproducibility. The planar lipid bilayer array formed by microfluidic technique is a planar lipid bilayer array formed by microfluidic technique (PDMS device) 1 saturated with water by preliminarily immersing in water, comprising microchannels 2 connected to an inlet of a microfluidic channel and arranged in parallel, and microchambers 3 having apertures on both sides of the microchannel 2.

Claims

1. A planar lipid bilayer array formed by microfluidic technique on a polydimethylsiloxane substrate saturated with water, wherein the polydimethylsiloxane substrate comprises:

(i) microchannels connected to an inlet of a microfluidic channel, and arranged in parallel; and

(ii) microchambers having apertures providing fluid communication with a microchannel, the microchambers being located on both sides of each of the microchannels; and

wherein the apertures of the microchambers are sealed with a planar lipid layer; and

wherein each of the microchannels is located between opposing walls of the substrate and apertures of the microchambers are located in both of the opposing walls of the substrate.

2. The planar lipid bilayer array formed by microfluidic technique according to claim 1, wherein the apertures on the both sides of the microchannel are arranged in a zigzag manner in relation to the microchannel.

3. The planar lipid bilayer array formed by microfluidic technique according to claim 1, wherein the microchannel is 7 μ

m in height, and the microchamber is 17 μ

m in width and 19 μ

m in height.