Selective extraction of glutelin proteins from freshwater or saltwater algae
First Claim
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1. A method of selectively extracting glutelin proteins from a freshwater or saltwater algal biomass or algal culture comprised of intact algal cells, the method comprising:
- heating the freshwater or saltwater algal biomass or algal culture to generate a heated extraction mixture comprising a liquid fraction and an algal biomass fraction, wherein the heating is to a temperature wherein the viscosity of the heated extraction mixture is reduced relative to the viscosity at room temperature, and the temperature is below the boiling point of the extraction mixture;
raising the pH of the heated extraction mixture above pH 9 to enrich the liquid fraction in glutelin proteins; and
separating the liquid fraction enriched in glutelin proteins from the algal biomass fraction.
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Abstract
Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
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5 Claims
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1. A method of selectively extracting glutelin proteins from a freshwater or saltwater algal biomass or algal culture comprised of intact algal cells, the method comprising:
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heating the freshwater or saltwater algal biomass or algal culture to generate a heated extraction mixture comprising a liquid fraction and an algal biomass fraction, wherein the heating is to a temperature wherein the viscosity of the heated extraction mixture is reduced relative to the viscosity at room temperature, and the temperature is below the boiling point of the extraction mixture; raising the pH of the heated extraction mixture above pH 9 to enrich the liquid fraction in glutelin proteins; and separating the liquid fraction enriched in glutelin proteins from the algal biomass fraction. - View Dependent Claims (2, 3, 4, 5)
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