Fluorescent phospholipase A 2 indicators
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Abstract
Compositions, methods of synthesis and applications of phospholipase A2 (PLA2) specific enzyme substrates which exhibit fluorescence resonance energy transfer (FRET) are described. The compounds generally have the structure: (I) wherein, the variables are described throughout the application. These novel compounds provide a sensitive method to monitor real time PLA2 specific enzyme activities in various cells, tissues and small organisms with fluorescence-ratiometric analysis.
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Citations
32 Claims
- 1. A compound of the formula:
- 12. A compound of formula:
- 24. A compound of formula:
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28. A method for detecting phospholipase A2 (PLA2) activity in a sample, comprising:
contacting the sample with a compound of formula; - View Dependent Claims (29, 30)
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31. A kit for detecting phospholipase A2 (PLA2) activity, comprising:
- a compound of formula;
- a compound of formula;
Specification