Electrode preconditioning

• US 8,518,236 B2
• Filed: 06/28/2006
• Issued: 08/27/2013
• Est. Priority Date: 06/29/2005
• Status: Expired due to Fees

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Roblin, Patricia Mary Elizabeth, Hyland, Mark, Newman, Christopher Paul
• Primary Examiner(s): Ball, J. Christopher
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US11/993,418
• Publication Number:
  US 20100140108A1
• Time in Patent Office: 2,617 Days
• Field of Search: 204280-296, 20440301-40315, 204/402, 204/157.61, 204/406, 204/434, 205/777.5, 205/778, 205/792, 205/775, 422 681- 98
• US Class Current: 205/775.5
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements

Abstract

A method for improving the precision of electrochemical measurements made using an electrochemical cell is provided. The method comprises preconditioning a working electrode of the cell by (i) baking the working electrode; and/or (ii) incubating the working electrode; and/or (iii) applying a preconditioning potential across the cell; and/or (iv) treating the working electrode with a UV laser.

Claims

1. A method of preparing an electrochemical device, said method comprising:

(a) providing an electrochemical cell having a working electrode and a pseudo reference electrode;

(b) preconditioning the working electrode by applying a stepped preconditioning potential across the cell for a duration of up to 100s, wherein the preconditioning potential is applied in the presence of an aqueous solution capable of allowing an electric current to pass and the preconditioning potential consists of either one potential step, or one positive and one negative potential step;

(c) providing an electroactive reagent to the electrochemical cell and optionally drying the reagent;

(d) supplying a sample comprising a bodily fluid to the device, the sample being in contact with the working electrode and with the electroactive reagent; and

(e) applying a measuring potential across the cell and measuring the resulting electrochemical response;

wherein the electrochemical response of the cell is not measured during the preconditioning step; and

wherein said preconditioning step (b) is carried out either before step (c) or between steps (c) and (d).

2. A method according to claim 1, wherein the electroactive reagent comprises an electrochemical mediator.

3. A method according to claim 2, wherein the mediator is a ruthenium complex or a ferricyanide or ferrocyanide salt.

4. A method according to claim 1, wherein the electroactive reagent is substantially free of metals capable of adsorbing to the working electrode.

5. A method according to claim 1, wherein the electroactive reagent does not contain cobalt.

6. A method according to claim 1, wherein the preconditioning potential is applied in the presence of an aqueous buffer which contacts the working and pseudo reference electrodes.

7. A method according to claim 1, wherein the preconditioning potential is applied by stepping the potential to a value of between −

0.1V and +1.8V.

8. A method according to claim 1, wherein the working electrode has at least one dimension of less than 50 μ

m.

9. A method according to claim 1, wherein the electrochemical cell is in the form of a receptacle, the working electrode is in a wall of the receptacle and the method comprises inserting the electroactive reagent into the receptacle.

10. A method according to claim 1, wherein the device comprises a strip having at least one receptacle or partial receptacle formed therein, the receptacle or partial receptacle having a first open part in a first surface of the strip to enable a sample to enter the receptacle or partial receptacle,

wherein the working electrode of the electrochemical cell is in a wall or walls of the receptacle or partial receptacle,

wherein the pseudo reference electrode of the electrochemical cell comprises a pseudo reference electrode layer formed on at least a part of the first surface of the strip, and

wherein the method comprises inserting the electroactive reagent into the receptacle.

11. A method according to claim 1, wherein the preconditioning potential is applied in the presence of an aqueous solution containing an anion which can be activated to form a per-derivative.

12. A method according to claim 1, wherein the preconditioning potential consists of a single potential step.

13. A method according to claim 1, wherein the preconditioning potential consists of one positive and one negative potential step.

14. A method according to claim 1, wherein said preconditioning step (b) is carried out before step (c).

15. A method of preparing an electrochemical device, said method comprising:

(a) providing an electrochemical cell having a working electrode and a pseudo reference electrode;

(b) treating the working electrode with a UV laser for a duration of at least 2s;

(c) providing an electroactive reagent to the electrochemical cell and optionally drying the reagent;

(d) supplying a sample comprising a bodily fluid to the device, the sample being in contact with the working electrode and with the electroactive reagent; and

(e) applying a measuring potential across the cell and measuring the resulting electrochemical response.

16. A method according to claim 15, wherein the electroactive reagent comprises an electrochemical mediator.

17. A method according to claim 16, wherein the mediator is a ruthenium complex or a ferricyanide or ferrocyanide salt.

18. A method according to claim 15, wherein the electroactive reagent is substantially free of metals capable of adsorbing to the working electrode.

19. A method according to claim 15, wherein the electroactive reagent does not contain cobalt.

20. A method according to claim 15, wherein the working electrode has at least one dimension of less than 50 μ

m.

21. A method according to claim 15, wherein the electrochemical cell is in the form of a receptacle, the working electrode is in a wall of the receptacle and the method comprises inserting the electroactive reagent into the receptacle.

22. A method according to claim 15, wherein the device comprises a strip having at least one receptacle or partial receptacle formed therein, the receptacle or partial receptacle having a first open part in a first surface of the strip to enable a sample to enter the receptacle or partial receptacle,

wherein the working electrode of the electrochemical cell is in a wall or walls of the receptacle or partial receptacle,

wherein the pseudo reference electrode of the electrochemical cell comprises a pseudo reference electrode layer formed on at least a part of the first surface of the strip, and

wherein the method comprises inserting the electroactive reagent into the receptacle.

23. A method according to claim 15, wherein the working electrode is preconditioned by applying a UV laser for a duration of at least 3s.

24. A method according to claim 15, wherein the working electrode is preconditioned by applying a pulsed UV laser operating at a pulse rate frequency (prf) of at least 25 kHz.