Stem cell capture and immobilization coatings for medical devices and implants

• US 8,518,431 B2
• Filed: 07/06/2012
• Issued: 08/27/2013
• Est. Priority Date: 08/07/2008
• Status: Expired due to Fees

• Current Assignee: Bioactive Surgical, Inc.
• Original Assignee: Bioactive Surgical, Inc.
• Inventors: Spedden, Richard H., Qiu, Judy, Borch, William
• Primary Examiner(s): HILL, KEVIN KAI
• Application Number:
  US13/543,059
• Publication Number:
  US 20120270294A1
• Time in Patent Office: 417 Days
• Field of Search: 424/93.1, 424/93.7, 424/423
• US Class Current: 424/423
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 29/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 29/048 Other specific proteins or polypeptides not covered by A61L29/045 - A61L29/047
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 31/047 Other specific proteins or polypeptides not covered by A61L31/044 - A61L31/046
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease

Abstract

Constructs and methods for immobilizing stem and other precursor cells, as well as other bioactive materials of therapeutic value on the surfaces of medical devices, such as bone, cartilage, spinal and tooth implants, are described herein. The present invention has broad application in the incorporation of bioactive and therapeutic materials in or on a medical implant or other interventional device, having particular value in enabling the real-time, utilization by medical personnel of bioactive materials extracted from the patient and subsequently reintroduced and immobilized in an implant device.

Claims

1. A method of constructing a target cell-carrying medical implant device, said method comprising the steps of:

a. providing a porous biocompatible medical implant comprising a solid exterior surface having a plurality of pores;

b. providing a solution comprised of the following components;

(i) a non-polar liquid, (ii) a polar liquid and (ii) a plurality of amphiphilic molecules composed of hydrophobic tail ends and hydrophilic head ends, wherein said head ends have a binding affinity for one or more target cells, further wherein said solution components assemble into non-polar-liquid-filled micelles or micelle-like constructs;

c. contacting said micelles or micelle-like constructs with a solution containing target cells to which the hydrophilic head ends of said amphiphilic molecules have a binding affinity and inducing said micelles or micelle-like constructs to bind said target cells and form micelle-target cell units; and

d. introducing said micelle-target cell units into the pores of said implant exterior surface so as to yield a medical implant device having target cells immobilized within said exterior surface pores.

2. The method of claim 1, wherein at least one of said hydrophilic head ends comprises a nucleic acid aptamer.

3. The method of claim 1, wherein said hydrophilic head ends comprise two or more nucleic acid aptamers having different structures and different binding affinities.

4. The method of claim 1, where said target cell is a stem cell.

5. The method of claim 1, wherein said solution of step (b) further comprises said target cells.

6. The method of claim 1, wherein step (c) is performed in an operating room environment.

7. The method of claim 1, wherein said target cells are autologous tissue of the patient.

8. The method of claim 1, further comprising the step of inducing a fraction of said micelle-target cell units to coalesce once introduced in the pores of said medical device through one or more factors selected from the group consisting of mechanical action, increased temperature, and changes in pH.

9. The method of claim 1, wherein said non-polar-liquid-filled micelles or micelle-like constructs comprise oil-in-water emulsions or water-in-oil emulsions.

10. The method of claim 9, wherein said medical device is selected from the list including a wound dressing, a tissue scaffold, a vertebral implant, a vascular implant, an intraluminal device, an anastomosis structure, a haemostatic barrier, a surgical suture and bone, cartilage and tooth implants.