Rapid detection of volatile organic compounds for identification of Mycobacterium tuberculosis in a sample
First Claim
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1. A method for identifying Mycobacterium tuberculosis bacteria in a sample, the method comprising:
- detecting one or more volatile organic compounds associated with Mycobacterium tuberculosis metabolism, wherein the volatile organic compounds are indicative of at least one of a presence of, response to treatment of, and resistance of the Mycobacterium tuberculosis bacteria in the sample, the one or more volatile organic compounds being selected from the group consisting of ethyl propionate, 1-pentanol, methyl valerate, 1-hexanol, ethyl valerate, methyl caproate, ethyl caproate, and any of the foregoing compounds in isotopically labeled form;
determining the amount of the one or more volatile organic compounds; and
identifying Mycobacterium tuberculosis in the sample based on presence and concentration of one or more volatile organic compounds.
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Abstract
In various embodiments, the invention relates to a method for identifying the presence of particular bacteria in a sample. The method includes collecting a sample that includes or has been exposed to the particular bacteria and detecting, in the sample, at least one volatile organic compound indicative of the presence of the bacteria.
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Citations
21 Claims
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1. A method for identifying Mycobacterium tuberculosis bacteria in a sample, the method comprising:
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detecting one or more volatile organic compounds associated with Mycobacterium tuberculosis metabolism, wherein the volatile organic compounds are indicative of at least one of a presence of, response to treatment of, and resistance of the Mycobacterium tuberculosis bacteria in the sample, the one or more volatile organic compounds being selected from the group consisting of ethyl propionate, 1-pentanol, methyl valerate, 1-hexanol, ethyl valerate, methyl caproate, ethyl caproate, and any of the foregoing compounds in isotopically labeled form; determining the amount of the one or more volatile organic compounds; and identifying Mycobacterium tuberculosis in the sample based on presence and concentration of one or more volatile organic compounds. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for identifying Mycobacterium tuberculosis bacteria in a sample, the method comprising:
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culturing a sample in a media comprising an alkali metal propionate, an alkali metal pentanoate, an alkali metal hexanoate, cholesterol, or any of the foregoing compounds in isotopically labeled form; detecting one or more volatile organic compounds associated with Mycobacterium tuberculosis metabolism indicating at least one of a presence of, response to treatment of, and resistance of the Mycobacterium tuberculosis bacteria from the cultured sample, the one or more volatile organic compounds being selected from the group consisting of ethyl propionate, 1-pentanol, methyl valerate, 1-hexanol, ethyl valerate, methyl caproate, ethyl caproate, and any of the foregoing compounds in isotopically labeled form; determining the amount of the one or more volatile organic compounds; and identifying Mycobacterium tuberculosis in the sample based on presence and concentration of one or more volatile organic compounds. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
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Specification