Method for measuring blood components and biosensor and measuring instrument for use therein

• US 8,524,055 B2
• Filed: 04/26/2011
• Issued: 09/03/2013
• Est. Priority Date: 04/19/2004
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Fujiwara, Masaki, Shinno, Teppei, Ikeda, Shin
• Primary Examiner(s): Ball, J. Christopher
• Assistant Examiner(s): DIETERLE, JENNIFER M
• Application Number:
  US13/094,356
• Publication Number:
  US 20110198223A1
• Time in Patent Office: 861 Days
• Field of Search: 20440301-40315, 422/82.01, 205/792, 205/777.5, 205/778, 600345-348, 435/287.1
• US Class Current: 204/403.11
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3274 Corrective measures, e.g. error detection, compensation for temperature or hematocrit, calibration coding of calibration information G01N33/48771
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/413 Concentration cells using liquid electrolytes measuring currents or voltages in voltaic cells
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

The present invention provides a method of measuring a component in blood, by which the amounts of blood cells and an interfering substance can be measured with high accuracy and high reliability and the amount of the component can be corrected accurately based on the amounts of the blood cells and the interfering substance. In a sensor for measuring a blood component, a first working electrode 13 measures a current that flows during a redox reaction of a blood component, a second working electrode 17 measures the amount of blood cells, and a third working electrode 12 measures the amount of an interfering substance. Next, based on the measurement results, the amount of the blood component to be measured is corrected. Thus, more accurate and precise measurement of the amount of the blood component can be realized.

Claims

1. A biosensor for measuring a component in blood,

wherein the biosensor comprises:

a first analysis portion comprising a first electrode system on which at least an oxidoreductase that acts upon the component and a mediator are provided, the first electrode system comprising a working electrode and a counter electrode;

a second analysis portion comprising a second electrode system that comprises a working electrode and a counter electrode, a mediator being provided on the counter electrode but not on the working electrode; and

a third analysis portion comprising a third electrode system that comprises a working electrode and a counter electrode,

in the first analysis portion, the component in the blood is measured by causing a redox reaction between the component and the oxidoreductase in the presence of the mediator and detecting with the first electrode system a redox current caused to flow when a voltage is applied,

in the second analysis portion, an amount of blood cells contained in the blood is measured by supplying the blood to the second electrode system, applying a voltage to the second electrode system in this state to cause a redox current to flow through the second electrode system, and detecting the redox current,

in the third analysis portion, an amount of an interfering substance contained in the blood is measured by supplying the blood to the third electrode system, applying a voltage to the third electrode system in this state to cause a redox current to flow through the third electrode system, and detecting the redox current, and

the working electrodes and the counter electrodes of the first, second and third electrode systems are made of gold, platinum, or palladium.

2. The biosensor according to claim 1, wherein, in the third electrode system, a mediator is provided at least on the counter electrode.

3. The biosensor according to claim 1, further comprising a channel for leading blood to the biosensor,

wherein the working electrode of the second analysis portion is located furthest upstream, and the first electrode system, the counter electrode of the second system and the third electrode system are located downstream with respect to flow of the blood supplied from a first end of the channel, or the working electrode of the third analysis portion is located furthest upstream and the first electrode system, and the second system and the counter electrode of the third electrode system are located downstream with respect to flow of the blood supplied from one end of the channel.

4. The biosensor according to claim 3, wherein the first analysis portion is located furthest downstream in the channel.

5. The biosensor according to claim 1, wherein at least one electrode selected from the working electrodes and the counter electrodes of the first electrode system, the second electrode system, and the third electrode system serves also as any of the other electrodes.

6. The biosensor according to claim 5, wherein a mediator is not provided on the working electrode of the third electrode system, and the working electrode of the second electrode serves also as the working electrode of the third electrode.

7. The biosensor according to claim 6, wherein either one or a combination of the electrodes of the first electrode system serves also as at least one of the second electrode system and the third electrode system as the counter electrode.

8. The biosensor according to claim 5, wherein the counter electrode of the second electrode system serves also as the working electrode of the third electrode system.

9. The biosensor according to claim 5, wherein the counter electrode of the second electrode system serves also as the working electrode of the third electrode system and the counter electrode of the third electrode system serves also as the counter electrode of the first electrode system.

10. The biosensor according to claim 1, further comprising a liquid detecting electrode,

wherein the liquid detecting electrode is located downstream from at least one of the analysis portions so that whether or not the blood is supplied to the at least one of the analysis portions can be detected with the liquid detecting electrode.