Generation of chemical building blocks from plant biomass by selective depolymerization

• US 8,524,471 B2
• Filed: 06/12/2008
• Issued: 09/03/2013
• Est. Priority Date: 03/19/2007
• Status: Expired due to Fees

• Current Assignee: Sued-Chemie IP GmbH & Co., KG (Clariant AG)
• Original Assignee: Sued-Chemie IP GmbH & Co., KG (Clariant AG)
• Inventors: Koltermann, Andre, Bruck, Thomas, Kettling, Ulrich, Rarbach, Markus
• Primary Examiner(s): Carlson, Karen Cochrane
• Assistant Examiner(s): MOSS, NATALIE M
• Application Number:
  US12/138,096
• Publication Number:
  US 20090004697A1
• Time in Patent Office: 1,909 Days
• Field of Search: 435/105, 435/71.1, 435/4
• US Class Current: 435/105
• CPC Class Codes: C12N 9/0061 Laccase (1.10.3.2)
• CPC Class Codes: C12N 9/2434 acting on beta-1,4-glucosidic bonds
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/12 Disaccharides
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 19/16 produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin non-biological hydrolysis of starch C08B30/00
• CPC Class Codes: C12P 19/18 produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: C12P 19/22 produced by the action of a beta-amylase, e.g. maltose
• CPC Class Codes: C12P 19/24 produced by the action of an isomerase, e.g. fructose
• CPC Class Codes: C13B 35/005 using microorganisms or enzymes
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: C13K 1/06 obtained by saccharification of starch or raw materials containing starch
• CPC Class Codes: C13K 13/00 Sugars not otherwise provided for in this class
• CPC Class Codes: C13K 13/002 Xylose

Abstract

The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.

Claims

1. A method for the enzymatic treatment of raw polymeric feedstock comprising soluble and insoluble components, the method comprising:

a. treating the insoluble raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the raw polymeric feedstock; and

b. separating the defined soluble monomeric or oligomeric building blocks produced in a) from the remainder of the insoluble raw polymeric feedstock, wherein the raw polymeric feedstock comprises at least 1 wt-% lignin, and

wherein a) and b) are sequentially repeated one or more times with different enzyme systems in order to liberate from the remainder of the raw polymeric feedstock other defined soluble monomeric or oligomeric building blocks, and

wherein the enzyme system used in a particular enzymatic treatment contains not more than 20% of contaminating enzymatic activities, which can cause liberation of other defined monomeric or oligomeric building blocks, which have not been liberated in previous enzymatic treatments.

2. The method of claim 1, wherein the raw polymeric feedstock comprises at least 10 wt-% lignin.

3. The method of claim 1, wherein no ligninolytic enzyme treatment is performed.

4. The method of claim 1, wherein the content of lignin in the polymeric feedstock, calculated as wt-% of the overall composition of the polymeric feedstock is not reduced during a) and b) or their repetition.

5. The method of claim 1, wherein prior to a) soluble components are separated from the raw polymeric feedstock.

6. The method of claim 5, wherein the separation of soluble components from the raw polymeric feedstock is performed using one or more washings and/or and one or more physico-chemical treatments.

7. The method of claim 1, wherein the enzyme system used in a) contains not more than 5% of contaminating enzyme activities apart from an enzyme activity resulting in liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a).

8. The method according to claim 1, wherein prior to the second enzymatic treatment of a), the method further comprises separating the soluble components from the processed polymeric feedstock using one or more pretreatments, optionally one or more washings.

9. The method of claim 1, wherein the enzymatic treatment is performed in an aqueous medium, said defined monomeric or oligomeric building blocks liberated from the raw or processed polymeric feedstock are soluble in the aqueous medium and the separation according to b) is performed by liquid/solid separation of the soluble building blocks in the aqueous medium from the remainder of the insoluble raw or processed polymeric feedstock.

10. The method of claim 1, wherein the defined monomeric or oligomeric building blocks according to a) are chosen from one of the group consisting of glucose, xylose, arabinose, galactose, mannose, amino acids and phenolic compounds.

11. The method of claim 1, wherein the feedstock is a cellulose-xylan-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a glucose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is glucose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a xylose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is xylose.

12. The method according to claim 1 wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Beta-glucosidase from A. niger or T. reesei;

Cellobiohydrolase I-II from T. reesei, and Endo-beta-1-4-D -glucanase I-V from T. reesei, and wherein the enzyme or enzyme system used in the second treatment of a) is selected from the group consisting of Endoxylanase from A. niger or T. reesei or C. thermocellum; and

Xylosidase from A. niger or T. reesei.

13. The method of claim 1, wherein the feedstock is a cellulose-xylan-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a xylose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is xylose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a glucose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is glucose.

14. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endoxylanase from A. niger or T. reesei or C. thermocellum, and Xylosidase from A. niger or T. reesei, and wherein the enzyme or enzyme system used in the second treatment of a) is selected from the group consisting of Beta-glucosidase from A. niger or T. reesei;

Cellobiohydrolase I-II from T. reesei, and Endo-beta-1-4-D -glucanase I-V from T. reesei.

15. The method of claim 1, wherein the feedstock is an arabinan-pectin-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a arabinose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is arabinose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a uronic acid-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is uronic acid.

16. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endoarabinase from A. niger and Arabinofucosidase from A. niger, and wherein the enzyme or enzyme system used in the second treatment of a) is a Pectinase from A. aculeatus, A. niger or C. japonicus.

17. The method of claim 1, wherein the feedstock is an arabinan-pectin-cellulose-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is an arabinose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is arabinose, wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a glucose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is glucose, and wherein the enzyme or enzyme system used in the third enzymatic treatment of a) is a uronic acid-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is uronic acid.

18. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endoarabinase from A. niger and Arabinofucosidase from A. niger, wherein the enzyme or enzyme system used in the second treatment of a) is selected from the group consisting of Beta-glucosidase from A. niger or T. reesei, Cellobiohydrolase I-II from T. reesei, and Endo-beta-1-4-D -glucanase I-V from T. reesei, and wherein the enzyme or enzyme system used in the third treatment of a) is a Pectinase from A. aculeatus, A. niger or C. japonicus.

19. The method of claim 1, wherein the feedstock is a galactan-pectin-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a galactose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is galactose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a uronic acid-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is uronic acid.

20. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endogalactonase from A. niger or C. thermocellum, and beta-Galactosidase from A. niger or K. fragilis, and wherein the enzyme or enzyme system used in the second treatment of a) is a Pectinase from A. aculeates, A. niger or C. japonicus.

21. The method of claim 1, wherein the feedstock is a mannan-xylan-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a mannose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is mannose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a xylose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is xylose.

22. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endo-Mannanase from A. niger, B. subtilis, T. maritima or T. reesei, and Exo-Mannosidase from C. fimi, and wherein the enzyme or enzyme system used in the second treatment of a) is selected from the group consisting of Endoxylanase from A. niger or T. reesei or C. thermocellum, and Xylosidase from A. niger or T. reesei.

23. The method of claim 1, wherein the feedstock is a mannan-cellulose-rich feedstock and wherein the enzyme or enzyme system used in the first enzymatic treatment of a) is a mannose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is mannose, and wherein the enzyme or enzyme system used in the second enzymatic treatment of a) is a glucose-liberating enzyme or enzyme system, and the defined soluble monomeric or oligomeric building block is glucose.

24. The method of claim 1, wherein the enzyme or enzyme system used in the first treatment of a) is selected from the group consisting of Endo-Mannanase from A. niger, B. subtilis, T. maritima or T. reesei, and Exo-Mannosidase from C. fimi, and wherein the enzyme or enzyme system used in the second treatment of a) is selected from the group consisting of Beta-glucosidase from A. niger or T. reesei, Cellobiohydrolase I-II from T. reesei, and Endo-beta-1-4-D -glucanase I-V from T. reesei.

25. The method of claim 1, wherein the raw polymeric feedstock comprises cellulose and hemicellulose and the enzyme system used in a particular enzymatic treatment has cellulase activity, and optionally beta-glycosidase, glucohydrolase or alpha- or beta-amylase activity, but is essentially free of hemicellulase activity.

26. The method of claim 1, wherein the enzyme system used in a particular enzymatic treatment contains as contaminating enzymatic activities one or more of such enzymatic activities which have been employed in a previous enzymatic treatment using a different enzyme system or which can only cause liberation of other monomeric or oligomeric building blocks from polymeric feedstock that are initially essentially absent in the raw polymeric feedstock.

27. The method of claim 1, wherein the enzyme system used in a particular enzymatic treatment has a first enzymatic activity and contains at least one additional enzymatic activity, which leads to the same defined monomeric or oligomeric building block from the raw or processed polymeric feedstock as the first enzymatic activity of the enzyme system.

28. The method of claim 1, wherein the insoluble raw or processed polymeric feedstock is subjected to a selective or unselective physico-chemical treatment prior to a) or prior to repetition of a).

29. The method according to claim 28, wherein the physico-chemical treatment comprises a treatment with aqueous solvents, organic solvents, or any combination or mixtures of these with ethanol or glycerol.

30. The method according to any one of claims 16, 18, and 20, wherein the Pectinase is pectate lyase.

31. The method according to any one of claims 16, 18, and 20, wherein the Pectinase is polygalactourenase.

32. The method of claim 1, wherein the enzyme system used in a) comprises not more than 10% of contaminating enzyme activities apart from an enzyme activity resulting in liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a).

33. The method of claim 1, wherein the enzyme system used in a) comprises not more than 2% of contaminating enzyme activities apart from an enzyme activity resulting in liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a).

34. The method of claim 1, wherein the enzyme system used in a) comprises not more than 1% of contaminating enzyme activities apart from an enzyme activity resulting in liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a).

35. The method according to claim 1, wherein the enzyme system used in a) comprises an enzyme activity resulting in the liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a), wherein said enzyme activity is at least 99% of all enzyme activities present in the enzyme system.

36. The method according to claim 1, wherein the enzyme system used in a) comprises an enzyme activity resulting in the liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a), wherein said enzyme activity is at least 95% of all enzyme activities present in the enzyme system.

37. The method according to claim 1, wherein the enzyme system used in a) comprises an enzyme activity resulting in the liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a), wherein said enzyme activity is at least 90% of all enzyme activities present in the enzyme system.

38. The method according to claim 1, wherein the enzyme system used in a) comprises an enzyme activity resulting in the liberation of said defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock according to a), wherein said enzyme activity is at least 80% of all enzyme activities present in the enzyme system.