Method of making a paired tag library for nucleic acid sequencing

• US 8,530,197 B2
• Filed: 01/08/2009
• Issued: 09/10/2013
• Est. Priority Date: 01/09/2008
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Li, Bin, Xi, Lei, Ranade, Swati S., Wang, Yangzhou
• Primary Examiner(s): WILDER, CYNTHIA B
• Application Number:
  US12/350,837
• Publication Number:
  US 20090181861A1
• Time in Patent Office: 1,706 Days
• Field of Search: 435/91.2, 435/6.1
• US Class Current: 435/91.2
• CPC Class Codes: C12N 15/1093 General methods of preparing gene libraries, not provided for in other subgroups
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2521/325 Single stranded exonuclease
• CPC Class Codes: C12Q 2525/191 incorporating an adaptor
• CPC Class Codes: C12Q 2525/307 Circular oligonucleotides

Abstract

The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Claims

1. A method of forming a paired tag comprising a first tag sequence and a second tag sequence that together comprise at least part of a polynucleotide of interest, said method comprising:

(a) ligating a first end and a second end of a double stranded polynucleotide of interest to one or more double stranded nucleic acid adaptors to form a circular double stranded nucleic acid molecule having a first nick and a second nick on different strands of the circular nucleic acid molecule wherein a first strand of the first end of the double stranded polynucleotide of interest is joined to one strand of the one or more double stranded nucleic acid adaptors so as to form a first nick, and wherein a second strand of the second end of the double stranded polynucleotide of interest is joined to one strand of the one or more double stranded nucleic acid adaptors so as to form a second nick; and

(b) performing a nick translation reaction to move the first and the second nick to new positions within the double stranded polynucleotide of interest.

2. The method of claim 1, further comprising:

allowing the nick translation reaction to proceed for a specific time; and

terminating the nick translation reaction.

3. The method of claim 1, wherein the first end and the second end of the double stranded polynucleotide of interest lack 5′

phosphate residues.

4. The method of claim 1, further comprising terminating the nick translation reaction before the first nick and the second nick are translated past each other.

5. The method of claim 1, wherein the first nick and the second nick are translated more than 10 bases.

6. The method of claim 4, wherein the first nick and the second nick are translated less than 500 bases.

7. The method of claim 1, wherein at least one strand of the one or more double stranded adaptors lacks a 5′

phosphate residue.

8. The method of claim 6, wherein the first nick and the second nick are translated less than 100 bases.

9. The method of claim 4, wherein the first nick and the second nick are translated between about 20 bases to about 50 bases.

10. The method of claim 1, wherein the first nick or the second nick is translated less than 500 bases.

11. The method of claim 9, wherein the first nick or the second nick is translated more than 27 bases and less than 500 bases.

12. The method of claim 10, wherein the first nick or the second nick is translated less than 200 bases.

13. The method of claim 1, wherein the first nick or the second nick is translated from 28 bases to about 50 bases.

14. The method of claim 1, wherein the circular nucleic acid molecule comprises a first adaptor ligated to a second adaptor and the first and second adaptors are ligated to the first end and the second end of the double stranded polynucleotide of interest.

15. The method of claim 14, wherein the circular nucleic acid molecule comprises a first nick between the first end of the double stranded polynucleotide of interest and the first adaptor and a second nick between the second end of the double stranded polynucleotide of interest and the second adaptor.

16. The method of claim 13, wherein the circular nucleic acid sequence comprises a gap that is filled in with nucleotides when the nick translation reaction is performed, thereby resulting in a nick.

17. The method of claim 1, wherein the polynucleotide of interest comprises from 50 to 2500 nucleotides.

18. The method of claim 16, wherein the polynucleotide of interest is subjected to a size selection technique to select for polynucleotides comprising from 50 to 2500 nucleotides prior to the ligating step.

19. The method of claim 1, wherein the one or more double stranded nucleic acid adaptors comprise a binding moiety.

20. The method of claim 1, wherein the nick translation reaction is performed using an enzyme selected from the group consisting of E. Coli DNA polymerase I, Taq DNA polymerase, Vent DNA polymerase, Klenow DNA polymerase I, and phi29 DNA polymerase and any combination thereof.

21. The method of claim 1, wherein the nick translation reaction is performed using a DNA polymerase having 5′

to 3′

exonuclease activity.

22. The method of claim 1, wherein the nick translation reaction comprises removal of nucleotides by 5′

to 3′

exonuclease activity.

23. The method of claim 1, wherein the nick translation reaction occurs by strand displacement.

24. The method of claim 1, further comprising cleaving the circular nucleic acid molecule at a first translated nick, thereby forming a linear polynucleotide comprising a first tag sequence and a second tag sequence separated by the one or more double stranded nucleic acid adaptors, wherein the first tag sequence and the second tag sequence are derived from the polynucleotide of interest.

25. The method of claim 23, further comprising cleaving the circular nucleic acid molecule at a second translated nick.

26. The method of claim 23, wherein the circular nucleic acid molecule is cleaved by an enzyme selected from the group consisting of S1 nuclease, mung bean nuclease, nuclease P1, nuclease BAL-31, and any combination thereof.

27. The method of claim 23, further comprising amplifying the linear polynucleotide.

28. The method of claim 1, wherein the one or more double stranded nucleic acid adaptors comprise a binding moiety that comprises biotin.

29. The method of claim 28, further comprising the step of using streptavidin to purify the paired tag.

30. The method of claim 1, further comprising treating the polynucleotide of interest with an alkaline phosphatase prior to ligation, wherein a 5′

-phosphate is removed from both the first end and the second end of the polynucleotide of interest.

31. The method of claim 29, wherein the alkaline phosphatase is selected from the group consisting of calf intestinal alkaline phosphatase, bacterial alkaline phosphatase, shrimp alkaline phosphatase, and some combination thereof.

32. The method of claim 1, wherein the circular double stranded nucleic acid molecule comprises a first and a second adaptor, wherein the second adaptor is positioned adjacent to each other.

33. The method of claim 31, wherein one strand of the first adaptor and one strand of the second adaptor lack a 5′

phosphate.

34. The method of claim 1, wherein each step is performed in the order listed in claim 1.

71. The method of claim 1, wherein the circular nucleic acid molecule comprises a first adaptor ligated to the first end and the second end of a double stranded polynucleotide of interest.

72. The method of claim 71, wherein the circular nucleic acid molecule comprises a first nick between the first end of the double stranded polynucleotide of interest and the first adaptor and a second nick between the second end of the double stranded polynucleotide of interest and the first adaptor.

35. A method of forming a released paired tag from a DNA fragment of interest, comprising:

ligating a first adaptor to a first end of a DNA fragment of interest and ligating a second adaptor to a second end of the DNA fragment of interest, thereby producing an adaptor modified fragment, circularizing the adaptor modified fragment by attaching a third adaptor to the adaptor modified fragment, thereby forming a circular nucleic acid molecule wherein a first nick is present between the third adaptor and the first adaptor and a second nick is present between the second adaptor and the third adaptor, wherein the circular nucleic acid molecule comprises a first strand and a second strand of DNA, and wherein the first nick and the second nick are not present on the same strand of the circular nucleic acid molecule;

performing a nick translation reaction wherein a nick on each strand of the circular nucleic acid molecule is translated into the DNA fragment of interest, and cleaving the circular nucleic acid molecule at a position at a translated nick, thereby forming a released paired tag.

36. The method of claim 35, further comprising:

allowing the nick translation reaction to proceed for a specific time; and

terminating the nick translation reaction.

37. The method of claim 36, further comprising terminating the nick translation reaction prior to cleaving the polynucleotide strand at each position at a translated nick.

38. The method of claim 35, further comprising attaching primer adaptors to the ends of the paired tag.

39. The method of claim 38, further comprising amplifying the paired tag with primers for the primer adaptors.

40. The method of claim 39, wherein the amplification is a clonal amplification.

41. The method of claim 40, wherein the clonal amplification comprises emulsion polymerase chain reaction (emulsion PCR).

42. The method of claim 40, wherein the clonal amplification comprises bridge polymerase chain reaction (bridge PCR).

43. The method of claim 35, wherein the third adaptor comprises a binding moiety, and further comprising binding the binding moiety to a solid support.

44. The method of claim 42, wherein the solid support is an array.

45. The method of claim 35, further comprising sequencing a portion of the paired tag.

46. A method of forming a paired tag comprising a first tag sequence and a second tag sequence that together comprise at least part of a polynucleotide of interest, said method comprising:

ligating a first end of a double stranded polynucleotide of interest to at least one adaptor;

ligating a second end of the double stranded polynucleotide of interest to at least one adaptor;

forming a circular nucleic acid molecule comprising the double stranded polynucleotide of interest, a first nick comprising a first 3′

end on a first strand of the circular nucleic acid molecule and a second nick comprising a second 3′

end on a second strand of the circular nucleic acid molecule, wherein the first nick and the second nick are on different strands of the circular nucleic acid molecule;

extending the first 3′

end of the first strand of the circular nucleic acid molecule into a sequence of the double stranded polynucleotide of interest; and

extending the second 3′

end of the second strand of the circular nucleic acid molecule into a sequence of the double stranded polynucleotide of interest.

47. The method of claim 46, wherein prior to extending the first 3′

end, the first nick is located between the first end of the double stranded polynucleotide of interest and the at least one adaptor and the second nick is located between the second end of the double stranded polynucleotide of interest and the at least one adaptor.

48. The method of claim 47, wherein the first and second ends of the double stranded polynucleotide of interest are connected to a same adaptor.

49. The method of claim 46, wherein the adaptor comprises a first and a second adaptor, wherein the first adaptor is located on the first end of the double stranded polynucleotide of interest and wherein the second adaptor is located on the second end of the double stranded polynucleotide of interest upon formation of the circular nucleic acid molecule.

50. The method of claim 49, wherein the first adaptor is connected to the second adaptor through a linking polynucleotide.

51. The method of claim 50, wherein upon formation of the circular nucleic acid molecule, the first nick is located at an end of the first adaptor that is distal to an end of the first adaptor that is connected to the double stranded polynucleotide of interest and the second nick is located at an end of the second adaptor that is distal to an end of the second adaptor that is connected to the double stranded polynucleotide of interest.

52. The method of claim 51, wherein the adaptors are ligated to a linking polynucleotide before the adaptors are ligated to the double stranded polynucleotide of interest.

53. The method of claim 46, further comprising digesting at least a part of the first strand of the circular nucleic acid molecule and digesting at least a part of the second strand of the circular nucleic acid molecule, wherein the digesting results in at least a portion of the double stranded polynucleotide of interest becoming single stranded, wherein the digesting occurs before the extending of the first and second 3′

ends.

54. The method of claim 53, wherein a 5′

to 3′

exonuclease is used to digest said nicked circular nucleic acid molecule.

55. The method of claim 53, wherein the digestion is halted before the double stranded polynucleotide of interest becomes single stranded in its entirety.

56. The method of claim 53, wherein the entire double stranded polynucleotide of interest is digested to a single stranded polynucleotide of interest.

57. The method of claim 53, wherein a DNA polymerase is used to extend said nicked circular nucleic acid molecule.

58. The method of claim 46, further comprising digesting at least a part of the first strand of the circular nucleic acid molecule and digesting at least a part of the second strand of the circular nucleic acid molecule, wherein the digesting results in at least a portion of the double stranded polynucleotide of interest becoming single stranded, wherein the digesting occurs at the same time as the extending of the first and second 3′

ends.

59. The method of claim 58, wherein the digesting and the extending take place in the same reaction tube.

60. The method of claim 46, wherein the extending of the first 3′

end of the first strand of the circular nucleic acid molecule into a sequence of the double stranded polynucleotide of interest is achieved by nick translation.

61. The method of claim 60, wherein the extending of the second 3′

end of the second strand of the circular nucleic acid molecule into a sequence of the double stranded polynucleotide of interest is achieved by nick translation.

62. The method of claim 61, wherein the first and second nicks are expanded into a first gap and second gap respectively.

63. The method of claim 62, wherein an exonuclease is used to expand the first and second nicks into the first gap and second gap respectively.

64. The method of claim 63, wherein the exonuclease comprises a T7 exonuclease.

65. The method of claim 46, further comprising performing a single strand dependent digest to release the paired DNA tag.

66. A method of preparing a paired tag, said method comprising:

providing a nicked linking polynucleotide comprising;

a first adaptor comprising a first adaptor strand that is hybridized to a second adaptor strand, wherein the first adaptor strand lacks a phosphate group on its 5′

end;

a second adaptor comprising a third adaptor strand that is hybridized to a fourth adaptor strand, wherein the third adaptor strand lacks a phosphate group on its 5′

end; and

a linking polynucleotide comprising a first linking strand hybridized to a second linking strand, wherein the linking polynucleotide comprises a first end and a second end, wherein the first adaptor is attached to the first end of the linking polynucleotide such that a nick is present where the first adaptor strand lacks a phosphate group on its 5′

end, wherein the second linking strand is attached to the second adaptor strand, wherein the second adaptor is attached to the second end of the linking polynucleotide such that a nick is present where the third adaptor strand of the second adaptor lacks a phosphate group on its 5′

end, wherein the second strand of the linking polynucleotide is attached to the fourth adaptor strand;

ligating a first end of a double stranded polynucleotide of interest to the first adaptor and ligating a second end of the double stranded polynucleotide of interest to the second adaptor; and

moving the first and second nicks into the double stranded polynucleotide of interest.

67. The method of claim 66, further comprising a single strand dependent digest to linearize the nicked linking polynucleotide.

68. The method of claim 66, further comprising ligating an internal adaptor into the linearized nicked linking polynucleotide.

69. The method of claim 68, further comprising amplifying at least one strand of the double stranded polynucleotide of interest.

70. The method of claim 69, wherein the amplification is a PCR based amplification.