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Method of making a paired tag library for nucleic acid sequencing

  • US 8,530,197 B2
  • Filed: 01/08/2009
  • Issued: 09/10/2013
  • Est. Priority Date: 01/09/2008
  • Status: Active Grant
First Claim
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1. A method of forming a paired tag comprising a first tag sequence and a second tag sequence that together comprise at least part of a polynucleotide of interest, said method comprising:

  • (a) ligating a first end and a second end of a double stranded polynucleotide of interest to one or more double stranded nucleic acid adaptors to form a circular double stranded nucleic acid molecule having a first nick and a second nick on different strands of the circular nucleic acid molecule wherein a first strand of the first end of the double stranded polynucleotide of interest is joined to one strand of the one or more double stranded nucleic acid adaptors so as to form a first nick, and wherein a second strand of the second end of the double stranded polynucleotide of interest is joined to one strand of the one or more double stranded nucleic acid adaptors so as to form a second nick; and

    (b) performing a nick translation reaction to move the first and the second nick to new positions within the double stranded polynucleotide of interest.

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