Methods of determining analyte concentration
First Claim
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1. A method of determining a concentration of an analyte in blood, the method comprising steps of:
- stimulating a region of skin other than a fingertip by manually rubbing the exterior surface of the region for a duration of at least 1 second;
after stimulating the region of skin, creating an opening in the region of skin to cause a flow of blood from the region of skin;
coupling a electrochemical sensor strip to a meter, wherein the sensor strip comprises;
a sample chamber for holding blood, the sample chamber comprising a working electrode, wherein the sample chamber is sized to contain a volume of no more than about 1 μ
L of blood;
a first electrode and a second electrode disposed in the sample chamber and flanking the working electrode, wherein the first electrode indicates when the sample chamber is beginning to fill with sample, and the second electrode indicates when the sample chamber is substantially filled with sample; and
an analyte-responsive enzyme and a redox mediator disposed in the sample chamber;
contacting the sensor strip to the blood;
transporting no more than 1 microliter of the blood to the sample chamber via an un-pressurized mode of transport;
determining when the sample chamber is beginning to fill with sample;
determining when the sample chamber is substantially filled with sample; and
electrochemically determining the concentration of the analyte in the blood using the electrochemical sensor strip and meter.
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Abstract
A region of skin, other than the fingertips, is stimulated. After stimulation, an opening is created in the skin (e.g., by lancing the skin) to cause a flow of body fluid from the region. At least a portion of this body fluid is transported to a testing device where the concentration of analyte (e.g., glucose) in the body fluid is then determined. It is found that the stimulation of the skin provides results that are generally closer to the results of measurements from the fingertips, the traditional site for obtaining body fluid for analyte testing.
312 Citations
36 Claims
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1. A method of determining a concentration of an analyte in blood, the method comprising steps of:
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stimulating a region of skin other than a fingertip by manually rubbing the exterior surface of the region for a duration of at least 1 second; after stimulating the region of skin, creating an opening in the region of skin to cause a flow of blood from the region of skin; coupling a electrochemical sensor strip to a meter, wherein the sensor strip comprises; a sample chamber for holding blood, the sample chamber comprising a working electrode, wherein the sample chamber is sized to contain a volume of no more than about 1 μ
L of blood;a first electrode and a second electrode disposed in the sample chamber and flanking the working electrode, wherein the first electrode indicates when the sample chamber is beginning to fill with sample, and the second electrode indicates when the sample chamber is substantially filled with sample; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; contacting the sensor strip to the blood; transporting no more than 1 microliter of the blood to the sample chamber via an un-pressurized mode of transport; determining when the sample chamber is beginning to fill with sample; determining when the sample chamber is substantially filled with sample; and electrochemically determining the concentration of the analyte in the blood using the electrochemical sensor strip and meter. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of determining a concentration of an analyte in blood, the method comprising steps of:
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stimulating a region of skin other than a fingertip by manually rubbing the skin for a period of time adequate to obtain an analyte measurement that is at least 10% nearer a concentration determined from blood obtained from a fingertip measurement than a determination of the concentration of the analyte from blood obtained from the region of skin without stimulating; after stimulating the region of skin, lancing the region of skin to cause a flow of blood from the region; coupling an electrochemical sensor strip to a meter, wherein the sensor strip comprises; a sample chamber for holding blood, the sample chamber comprising a working electrode, wherein the sample chamber is sized to contain a volume of no more than about 1 μ
L of blood;a first electrode and a second electrode disposed in the sample chamber and flanking the working electrode, wherein the first electrode indicates when the sample chamber is beginning to fill with sample, and the second electrode indicates when the sample chamber is substantially filled with sample; and an analyte-responsive enzyme and a redox mediator disposed in the sample chamber; contacting the sensor strip to the blood and transporting no more than about 1 microliter of the blood to the sample chamber; determining when the sample chamber is beginning to fill with sample; determining when the sample chamber is substantially filled with sample; and electrochemically determining the concentration of the analyte by coulometry, amperometry or potentiometry using the electrochemical sensor strip and meter. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification