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Methods and apparatus for measuring analytes using large scale FET arrays

  • US 8,535,513 B2
  • Filed: 09/14/2012
  • Issued: 09/17/2013
  • Est. Priority Date: 12/14/2006
  • Status: Active Grant
First Claim
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1. A method for nucleic acid sequencing, comprising:

  • disposing a bead attached to at least one template nucleic acid in a reaction well communicating with a chemical sensor, wherein the chemical sensor comprises an ion-sensitive field-effect transistor (ISFET) including a floating gate coupled to the reaction well via a passivation layer;

    introducing a nucleotide into the reaction well;

    detecting an output pulse from the chemical sensor in response to a transient change in ion concentration within the reaction well pending incorporation of the nucleotide into the template nucleic acid; and

    determining at least a portion of a sequence corresponding to a portion of the template nucleic acid based on the detected output pulse.

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