Devices for and methods of monitoring a parameter of a fluidic sample by microdialysis

• US 8,535,537 B2
• Filed: 10/18/2007
• Issued: 09/17/2013
• Est. Priority Date: 10/19/2006
• Status: Expired due to Fees

• Current Assignee: JOANNEUM RESEARCH Forschungsgesellschaft mbH (Province of Styria)
• Original Assignee: JOANNEUM RESEARCH Forschungsgesellschaft mbH (Province of Styria)
• Inventors: Feichtner, Franz, Schaupp, Lukas, Koehler, Hans
• Primary Examiner(s): Kim, John
• Application Number:
  US12/446,234
• Publication Number:
  US 20110011786A1
• Time in Patent Office: 2,161 Days
• Field of Search: 210/321.62, 210/321.75, 210/321.84, 210/500.21, 210/500.24, 210/645, 210/646
• US Class Current: 210/645
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/686 Permanently implanted devices, e.g. pacemakers, other stimulators, biochips A61B5/6861 takes precedence
• CPC Class Codes: A61M 1/16 with membranes
• CPC Class Codes: A61M 1/1619 Sampled collection of used dialysate, i.e. obviating the need for recovery of whole dialysate quantity for post-dialysis analysis
• CPC Class Codes: A61M 1/1678 intracorporal peritoneal dialysis A61M1/28
• CPC Class Codes: A61M 1/3406 Physical characteristics of the filtrate, e.g. urea
• CPC Class Codes: A61M 2205/50 with microprocessors or computers
• CPC Class Codes: B01D 2313/086 Meandering flow path over the membrane
• CPC Class Codes: B01D 2325/022 Asymmetric membranes
• CPC Class Codes: B01D 2325/28 Degradation or stability over time
• CPC Class Codes: B01D 2325/48 Antimicrobial properties
• CPC Class Codes: B01D 63/02 Hollow fibre modules
• CPC Class Codes: B01D 63/031 Two or more types of hollow fibres within one bundle or within one potting or tube-sheet
• CPC Class Codes: B01D 63/087 Single membrane modules
• CPC Class Codes: B01D 69/02 characterised by their properties

Abstract

A device for monitoring a parameter of a fluidic sample by microdialysis, the device comprising a permeable membrane, wherein the permeable membrane has a first surface to be brought in contact with the fluidic sample to traverse the permeable membrane (300), wherein the permeable membrane has a second surface to be brought in contact with a dialysis fluid, and wherein the first surface is smoother than the second surface.

Claims

1. A microdialysis device for analyzing a fluidic sample, the microdialysis device comprising:

a permeable membrane;

wherein the permeable membrane has a first surface to be brought in contact with the fluidic sample to traverse the permeable membrane;

wherein the permeable membrane has a second surface to be brought in contact with a dialysis fluid;

wherein the first surface is smoother than the second surface so that a surface roughness of the first surface is smaller than a surface roughness of the second surface;

wherein the permeable membrane comprises pores having a mean size which increases from the first surface towards the second surface;

wherein the permeable membrane comprises a hollow tube an outer surface of the hollow tube forming the first surface;

wherein the pores have a size at the first surface smaller than essentially 0.1 μ

m;

wherein the pores have a size at the second surface in the range between essentially 0.1 μ

m and essentially 0.4 μ

m; and

wherein the permeable membrane has a molecular cut-off in a range between 1 kDa and 100 kDa.

2. The device of claim 1,

wherein the pores have a size at the first surface particularly in the range between essentially 0.05 μ

m and essentially 0.09 μ

m.

3. The device of claim 1,

wherein the permeable membrane has a molecular cut-off particularly in a range between 5 kDa and 50 kDa, more particularly of substantially 10 kDa.

4. The device of claim 1,

wherein the first surface is functionalized to maintain permeability of the permeable membrane for the fluidic sample when analyzing the fluidic sample.

5. The device of claim 4,

wherein the first surface is functionalized in a manner to suppress deposition and activation of components of the fluidic sample on the first surface.

6. The device of claim 1,

wherein the first surface is adapted to be brought in contact with blood, so that a part of the blood is enabled to traverse the permeable membrane.

7. The device of claim 1,

wherein the second surface is free of a functionalization.

8. The device of claim 1,

wherein the second surface is functionalized to maintain permeability of the permeable membrane for the fluidic sample when analyzing the fluidic sample and to prevent cell adhesion of sticky analytes to the second surface.

9. The device of claim 1,

adapted for vascular microdialysis.

10. The device of claim 1,

wherein the permeable membrane comprises pores having a mean size which increases continuously from the first surface towards the second surface.

11. The device of claim 1,

wherein the permeable membrane comprises pores having a mean size which increases in a stepwise manner from the first surface towards the second surface, particularly by manufacturing the permeable membrane using a plurality of layers, each layer having a specific mean pore size.

12. A method of monitoring a parameter of a fluidic sample by microdialysis, the method comprising:

bringing a first surface of a permeable membrane in contact with a fluidic sample to traverse the permeable membrane;

bringing a second surface of the permeable membrane in contact with a dialysis fluid;

wherein the first surface is smoother than the second surface so that a surface roughness of the first surface is smaller than a surface roughness of the second surface, the permeable membrane comprising a hollow tube, an outer surface of the hollow tube forming the first surface;

wherein the permeable membrane comprises pores having a mean size which increases from the first surface towards the second surface;

wherein the pores have a size at the first surface smaller than essentially 0.1 μ

m;

wherein the pores have a size at the second surface in the range between essentially 0.1 μ

m and essentially 0.4 μ

m; and

wherein the permeable membrane has a molecular cut-off in a range between 1 kDa and 100 kDa.

13. The method of claim 12, further comprising:

monitoring an analyte concentration, particularly for glucose monitoring.