×

Detection of disease related genes

  • US 8,535,885 B2
  • Filed: 04/02/2012
  • Issued: 09/17/2013
  • Est. Priority Date: 05/11/1994
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method, comprising:

  • a) providing;

    i) nucleic acid comprising a disease-related gene;

    ii) PCR primers capable of hybridizing to a portion of said disease-related gene so as to create amplified template;

    iii) transcription-translation reagents capable of producing nascent protein or protein fragments from said amplified template, said nascent protein or protein fragments comprising an affinity marker;

    iv) an acceptor molecule capable of binding to said affinity marker of said nascent protein;

    b) mixing said nucleic acid and said PCR primers under conditions such that said amplified template is created;

    c) contacting said amplified template with said transcription-translation reagents under conditions such that nascent protein or protein fragment is produced and said affinity marker is added to said nascent protein or fragment during translation; and

    d) reacting said acceptor molecule with said nascent protein or protein fragment.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×