×

Digital analyte analysis

  • US 8,535,889 B2
  • Filed: 02/11/2011
  • Issued: 09/17/2013
  • Est. Priority Date: 02/12/2010
  • Status: Active Grant
First Claim
Patent Images

1. A method for analyzing a target nucleic acid, the method comprising the steps of:

  • forming droplets containing a single target nucleic acid and one or more amplification reagents;

    amplifying the target in the droplets;

    distinguishing droplets containing amplicon from the target and amplicon from a variant of the target generated by polymerase error by using two differently labeled-hybridization probes, one hybridizing to the target and one hybridizing to a specific variant of the target; and

    analyzing target amplicons.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×