Method of measuring blood component, sensor used in the method, and measuring device
First Claim
1. A method of measuring a component in a blood sample, comprising:
- causing a redox reaction between the component in the blood sample and an oxidoreductase in the presence of a mediator;
detecting a first oxidation current or a reduction current caused by the redox reaction with an electrode system for measuring the component;
calculating an amount of the component based on a value of the detected current; and
measuring a Hct value of the blood sample and correcting the amount of the component using the measured Hct value, the measurement of the Hct value comprising;
applying a voltage in the presence of the blood sample to a second electrode system for Hct value measurement that comprises a counter electrode and a working electrode to cause an oxidation current or a reduction current to flow between the counter electrode and the working electrode for the Hct value measurement, the oxidoreductase and the mediator used for the redox reaction with the component in the blood sample being present on the counter electrode for the Hct value measurement, the counter electrode for the Hct value measurement being part of the first electrode system for detecting the oxidation or reduction current for measuring the component in the blood sample, and a mediator for the Hct value measurement being present on the counter electrode for Hct value measurement and not on the working electrode for Hct value measurement;
detecting the oxidation current or the reduction current from applying the voltage to the electrode system for Hct measurement; and
calculating the Hct value based on a value of the detected current.
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Abstract
The present invention provides a method of measuring a component in blood, by which an amount of the component can be corrected accurately by measuring a hematocrit (Hct) value of the blood with high accuracy and high reliability and also provides a sensor used in the method. The sensor for measuring a component in blood has a first analysis portion and a second analysis portion. The first analysis portion has a first electrode system (11,12) and a reagent layer (14), and the reagent layer (14) has an oxidoreductase that acts on the component and a mediator. In the first analysis portion, the component in the blood is measured by causing a redox reaction of the component with the oxidoreductase in the presence of the mediator and detecting a redox current caused when a voltage is applied by the first electrode (11,12). The second analysis portion has a working electrode and a counter electrode, and a mediator is provided on the counter electrode but not on the working electrode. In the second analysis portion, a Hct value of the blood is measured by supplying the blood to the electrode system, applying a voltage to cause a current to flow, and detecting a value of the current. Using this Hct value, the amount of the component is corrected.
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Citations
16 Claims
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1. A method of measuring a component in a blood sample, comprising:
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causing a redox reaction between the component in the blood sample and an oxidoreductase in the presence of a mediator; detecting a first oxidation current or a reduction current caused by the redox reaction with an electrode system for measuring the component; calculating an amount of the component based on a value of the detected current; and measuring a Hct value of the blood sample and correcting the amount of the component using the measured Hct value, the measurement of the Hct value comprising; applying a voltage in the presence of the blood sample to a second electrode system for Hct value measurement that comprises a counter electrode and a working electrode to cause an oxidation current or a reduction current to flow between the counter electrode and the working electrode for the Hct value measurement, the oxidoreductase and the mediator used for the redox reaction with the component in the blood sample being present on the counter electrode for the Hct value measurement, the counter electrode for the Hct value measurement being part of the first electrode system for detecting the oxidation or reduction current for measuring the component in the blood sample, and a mediator for the Hct value measurement being present on the counter electrode for Hct value measurement and not on the working electrode for Hct value measurement; detecting the oxidation current or the reduction current from applying the voltage to the electrode system for Hct measurement; and calculating the Hct value based on a value of the detected current. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification